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A novel previously undescribed fusarivirus from the phytopathogenic fungus Setosphaeria turcica

Archives of Virology volume 166, pages 665–669 (2021)Cite this article

Abstract

A putative mycovirus belonging to the proposed family “Fusariviridae” was discovered in Setosphaeria turcica by sequencing a double-stranded RNA extracted from this phytopathogenic fungus. The virus was tentatively named “Setosphaeria turcica fusarivirus 1” (StFV1). StFV1 has a genome comprising 6685 nucleotides. The genome contains three open reading frames (ORF). The largest ORF, ORF1, is preceded by an untranslated region (UTR) of 16 nucleotides and separated from ORF2 by an intergenic region of 63 nucleotides. The smallest ORF, ORF3, overlaps ORF2 by 16 nucleotides and is followed by a 3'-UTR of 82 nucleotides. The protein encoded by ORF1 is 71.8%, 67.4% and 68.1% identical to the RNA-dependent RNA polymerases (RdRps) of Pleospora typhicola fusarivirus 1 (PtFV1), Plasmopara viticola lesion-associated fusarivirus 1 (PvlaFV1), and Plasmopara viticola lesion-associated fusarivirus 3 (PvlaFV3), respectively, but has less than 47% amino acid sequence identity to the RdRps of other fusariviruses. To our knowledge, this is the first fusarivirus discovered in S. turcica and the first virus to be identified in this fungus using conventional cloning methods.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 31972243), the Science and Technology Project of Guizhou Tobacco Company (no. 201921f), and the Opening Fund of Key Lab of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University. We are grateful to Dr. Fangluan Gao and Dr. Zhenguo Du at the Institute of Plant Virology, Fujian Agriculture and Forestry University, for their help in preparing the manuscript.

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  1. Zhongnan Gao and Lina Cai contributed equally to this work.

Affiliations

  1. Hubei Engineering Research Center for Pest Forewarning and Management, Yangtze University, Jingzhou, 434025, Hubei, China

    Zhongnan Gao, Lina Cai, Hongliu An, Qingchao Deng, Songbai Zhang & Shouguo Fang

  2. Zunyi City Company, Guizhou Tobacco Company, Zunyi, 563000, Guizhou, China

    Minghong Liu & Xiaoyan Wang

  3. Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, 266101, Shandong, China

    Jinguang Yang

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  1. Zhongnan Gao
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  7. Qingchao Deng
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Correspondence to Songbai Zhang or Shouguo Fang.

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Gao, Z., Cai, L., Liu, M. et al. A novel previously undescribed fusarivirus from the phytopathogenic fungus Setosphaeria turcica. Arch Virol 166, 665–669 (2021). https://doi.org/10.1007/s00705-021-04954-x

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  • DOI: https://doi.org/10.1007/s00705-021-04954-x