Abstract
This work describes the first molecular characterization of grapevine virus A (GVA) in Turkish grapevine varieties based on the coat protein gene. RT-PCR detection revealed a high infection rate of GVA in two major viticultural areas, Eastern Mediterranean (EM) and Southeast Anatolia (SEA). The number of infected varieties was higher in SEA, where very ancient and traditional cultivars are in use and no foreign grapevine material has been introduced. High nucleotide and amino acid sequence similarity were seen between the Turkish GVA isolates and the reference isolates in group I and II. The viral isolates from the same location and cultivars were not phylogenetically related.
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Acknowledgements
The authors would like to thank Dr. A. Minafra for critically reading the manuscript and helpful suggestions.
Funding
This work was granted by the Research Fund of Kahramanmaraş Sütçü Imam University (project no: 2015/3-55YLS).
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Supplementary Fig. 2
Nucleotide sequence identity matrix of 22 GVA isolates from Turkey and eight representative isolates from the GenBank database, generated using Sequence Demarcation Tool (SDT) (DOC 68 KB)
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Balsak, S.C., Buzkan, N. Prevalence and genetic variability of grapevine virus A in Turkish autochthonous grapevine varieties. Arch Virol 166, 943–947 (2021). https://doi.org/10.1007/s00705-021-04953-y
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DOI: https://doi.org/10.1007/s00705-021-04953-y