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C1QBP inhibits proliferation of porcine circovirus type 2 by restricting nuclear import of the capsid protein

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Abstract

Complement component 1 Q subcomponent-binding protein (C1QBP) has been shown to interact with the porcine circovirus type 2 (PCV2) Cap protein. Here, using yeast two-hybrid (Y2H) and co-immunoprecipitation assays, as well as laser confocal microscopy, the interaction between C1QBP and Cap was confirmed. Furthermore, overexpression of C1QBP in cells altered the intracellular location of Cap, which was observed using confocal microscopy and verified by detection of Cap in nuclear protein extracts in a Western blot assay. By inhibiting nuclear transport of Cap, overexpression of C1QBP downregulated PCV2 proliferation in PK-15 cells, as determined by quantitative polymerase chain reaction (qPCR). As C1QBP plays a similar role in a fusion of green fluorescent protein (GFP) with the Cap nuclear localisation signal (NLS) sequence, (CapNLS-GFP), we propose that the target site for C1QBP in Cap is possibly located in the NLS region. Considering all the results together, this study demonstrated that C1QBP interacts with the Cap NLS region, resulting in changes in the intracellular localisation of the Cap protein. We confirmed that overexpression of C1QBP inhibits the proliferation of PCV2, and this is possibly related to the function of C1QBP in controlling nuclear transport of Cap.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31672581).

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  1. Xin Ma and Changjie Lv equal contribution.

Authors and Affiliations

  1. College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Xin Ma, Changjie Lv, Qianqian Wang, Chen Li, Peixin Wang, Chen Luo, Yifan Wu, Tingting Wei, Siying Liu, Fathalrhman Eisa Addoma Adam, Zengqi Yang & Xinglong Wang

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Ma, X., Lv, C., Wang, Q. et al. C1QBP inhibits proliferation of porcine circovirus type 2 by restricting nuclear import of the capsid protein. Arch Virol 166, 767–778 (2021). https://doi.org/10.1007/s00705-020-04950-7

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