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Diversity of enteric and non-enteric human adenovirus strains in Brazil, 2006-2011

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Abstract

During 2006-2011, 5035 fecal samples were tested by PCR for human adenovirus (HAdV) and sequenced. HAdV was detected in 198 cases (3.9%), with the highest rate in children ≤ 5 years. Enteric HAdVs were the most prevalent genotypes (78%; 146/187): HAdV-F41 (63.6%; 119/187), HAdV-F40 (12.3%; 23/187), HAdV-A12 (1.6%; 3/187) and HAdV-A31 (0.5%; 1/187). Non-enteric HAdVs were detected in 22% (41/187): HAdV-C1 (8.0%; 15/187), HAdV-C2 (6.9%; 13/187), HAdV-C5 (4.3%; 8/187), HAdV-D8 (1.3%; 2/187), HAdV-B21 (0.5%; 1/187), HAdV-B3 (0.5%; 1/187) and HAdV-C6 (0.5%; 1/187). This 6-year retrospective study points out a high diversity of HAdV types circulating in Brazil and highlights the need to carry out molecular epidemiological studies of HAdV among patients with acute diarrheal infection on a regular basis.

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Acknowledgements

We thank the Enteric Diseases Laboratory of Adolfo Lutz Institute staff for technical assistance: Antonio Erculiani Junior, Audrey Cilli, Giselle Aparecida Schiavelli, Rita de Cassia Compagnoli Carmona, Simone Guadagnucci Morillo and Sirlene Henrique Rodrigues Silva. We are grateful to Centers for Surveillance (CVE), São Paulo State Health Department; Public Health Laboratories (LACENs) and CGLAB/DEVEP/SVS/Ministry of Health, Brasília, for assistance in sample collection and epidemiological data.

Funding

Adriana Luchs is funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico/CNPq grant #400450/2016-0. Ellen Viana de Souza and Roberta Salzone Medeiros are supported by a fellowship of Institutional Program for Scientific Initiation Scholarships (Programa Institucional de Bolsas de Iniciação Científica/PIBIC-CNPq). Yasmin França Viana Pires de Souza is supported by a fellowship of Scientific Initiation (Iniciação Científica/CNPq) grant #400450/2016-0. Lais Sampaio de Azevedo and Talita Gonçalves Aires de Queiroz are supported by a fellowship of Training Program for Scientific Research at Instituto Adolfo Lutz (Programa de Formação para Investigação Científica do Instituto Adolfo Lutz/FEDIAL).

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Authors and Affiliations

  1. Enteric Disease Laboratory, Virology Center, Adolfo Lutz Institute, Av. Dr Arnaldo, nº 355, São Paulo, SP, 01246-902, Brazil

    Ellen Viana de Souza, Yasmin França Viana Pires de Souza, Roberta Salzone Medeiros, Lais Sampaio de Azevedo, Talita Gonçalves Aires de Queiroz, Maria do Carmo Sampaio Tavares Timenetsky & Adriana Luchs

  2. Retrovirology Laboratory, Federal University of São Paulo, São Paulo, Brazil

    Rodrigo Lopes Sanz-Duro, Robson dos Santos Souza Marinho & Shirley Vasconcelos Komninakis

  3. Postgraduate Program in Health Science, Faculty of Medicine of ABC, Santo André, Brazil

    Shirley Vasconcelos Komninakis

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  1. Ellen Viana de Souza
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Contributions

AL conceived the study; AL and MCSTT designed the study protocol; EVS, YFVPS, RSM, LSA, TGAQ and AL participated in the conduct of the study; EVS, YFVPS, RSM, LSA, TGAQ and AL acquired the data and performed the PCR assays; EVS, YFVPS, RSM, LSA, TGAQ, RLSD, RSSM, SVK and AL conducted the sequencing assays; EVS, YFVPS, RSM, LSA, TGAQ and AL analyzed and interpreted the data; AL drafted the manuscript; EVS, YFVPS, RSM, LSA, TGAQ, RLSD, RSSM, SVK and MCSTT critically revised the manuscript for intellectual content. All authors read and approved the final version. AL is the guarantor of the paper.

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Correspondence to Adriana Luchs.

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All author(s) declare that they have no conflict of interest.

Ethical approval

This study was carried out in accordance with the Declaration of Helsinki as revised in 2000 and approved by the Ethics Committee of the Adolfo Lutz Institute, São Paulo, Brazil (CTC 42-H/2015; CEP 1624723). Study participants were not required to provide informed consent, as this study was considered by the Ethics Committee to be part of routine surveillance activities.

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Souza, E.V., de Souza, Y.F.V.P., Medeiros, R.S. et al. Diversity of enteric and non-enteric human adenovirus strains in Brazil, 2006-2011. Arch Virol 166, 897–903 (2021). https://doi.org/10.1007/s00705-020-04946-3

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