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Chemiluminescent-microparticle-immunoassay-based detection and prevalence of human immunodeficiency virus infection in Islamabad, Pakistan

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Abstract

An advanced detection assay is important for clinical diagnosis of human immunodeficiency virus (HIV) infection in asymptomatic individuals. The first step in timely treatment and management of the infection is the early detection of HIV. In this study, we determined the prevalence of HIV in Islamabad for the first time using a chemiluminescent microparticle immunoassay (CMIA), which is an advanced serological diagnostic technique. A total of 3659 samples were obtained from the general public in Islamabad, Pakistan, and 85 of them were found to be positive for HIV infection (2.32% prevalence). Of the positive subjects, 78.82% (67/85) were male, 10.6% (9/85) were female, and 10.6% (9/85) were of unidentified gender. The results revealed a significant relationship between age groups and HIV status. The age group of 21- to 30-year-olds was found to have the highest rate of HIV infection, and the rate of HIV infection in males was higher than in females.

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Acknowledgment

The authors are thankful to the staff of Islamabad Diagnostics Center, Pakistan, for their support throughout the study.

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Authors and Affiliations

  1. Department of Biotechnology, Quaid-i-Azam University Islamabad, Islamabad, Pakistan

    Misbahud Din & Muhammad Ali

  2. Department of Genetics, Hazara University Mansehra, Mansehra, Pakistan

    Faheem Anwar

  3. Department of Statistics, Quaid-i-Azam University Islamabad, Islamabad, Pakistan

    Muhammad Yousaf

  4. Department of Bioinformatics and Biotechnology, International Islamic University Islamabad, Islamabad, Pakistan

    Bilal Ahmad

  5. Department of Microbiology and Biotechnology, Abasyn University Peshawar, Peshawar, Pakistan

    Abdullah

Authors
  1. Misbahud Din
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  2. Faheem Anwar
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  3. Muhammad Ali
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  4. Muhammad Yousaf
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  5. Bilal Ahmad
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  6. Abdullah
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Correspondence to Faheem Anwar or Muhammad Ali.

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Handling Editor: Carolina Scagnolari.

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Din, M., Anwar, F., Ali, M. et al. Chemiluminescent-microparticle-immunoassay-based detection and prevalence of human immunodeficiency virus infection in Islamabad, Pakistan. Arch Virol 166, 581–586 (2021). https://doi.org/10.1007/s00705-020-04897-9

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  • DOI: https://doi.org/10.1007/s00705-020-04897-9

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