Abstract
In the present study, the utility of viral RNA isolated from whole blood over plasma for detection of dengue virus (DENV) was investigated in 80 samples referred for serotyping by DENV serotype-specific one-step real-time RT-PCR. DENV RNA was detected in 71.25% of the whole blood samples compared to 46.25% in the corresponding plasma samples. In secondary infections, DENV RNA was detected in 83.3% of whole blood samples, while it was detected in 40.5% of plasma samples (P = 0.0001). Non-structural protein 1 (NS1) antigen was detected in only 54.8% of the secondary infections. The detection rate of DENV RNA in whole blood is higher than in plasma. We suggest that one-step real-time RT-PCR using RNA from whole blood combined with an NS1 ELISA should be the choice for dengue diagnosis in dengue vaccine trials.
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KA, DP and PSS conceived and designed the study. MBK, RVB, and MB performed the study. KA did the statistical analysis and wrote the manuscript. DP critically reviewed the manuscript, and all of the authors approved the content.
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Alagarasu, K., Kakade, M.B., Bachal, R.V. et al. Use of whole blood over plasma enhances the detection of dengue virus RNA: possible utility in dengue vaccine trials. Arch Virol 166, 587–591 (2021). https://doi.org/10.1007/s00705-020-04892-0
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DOI: https://doi.org/10.1007/s00705-020-04892-0