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Molecular epidemiology of GII noroviruses in outpatients with acute gastroenteritis in Shandong Province, China

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Abstract

Noroviruses have been recognized as the most important causative agents of acute gastroenteritis. The present study was carried out to investigate the molecular epidemiological features of genotype II (GII) norovirus in outpatients with acute gastroenteritis in Shandong province in China from July 2017 to June 2018. In total, 151 (10.30%) samples were positive for NoV GII strains by RT-PCR. Eight genotypes were detected: GII.2, GII.3, GII.4, GII.6, GII.7, GII.12, GII.13 and GII.17. GII.4 (43.71%) was the most prevalent genotype, and the dominant strains belonged to the group of Sydney-2012 strains. GII.17 (27.15%), which has become the main cause of outbreaks of acute gastroenteritis in China, also accounted for a high proportion. Meanwhile, three recombinant types (GII.P17-GII.7, GII.P3-GII.4 and GII.P12-GII.4) were observed and authenticated using Simplot software. The results showed that GII norovirus was the main cause of acute gastroenteritis in Shandong province. GII.4 and GII.17 were the dominant genotypes. Continuous observation and identification of emerging genotypes are necessary for understanding the evolution of the virus, control of infection, and development of vaccines.

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Acknowledgements

This study was funded by the Shandong Provincial Key Research and Development Program (2018 GSF118071).

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Author notes

  1. Chengxi Sun and Yingjie Zhao are co-first authors.

Authors and Affiliations

  1. Department of Clinical Laboratory, Cheeloo College of Medicine, Qilu Hospital, Shandong University, Wenhua Xi Road 107, Jinan, 250012, Shandong, China

    Chengxi Sun

  2. Department of Rheumatology, Cheeloo College of Medicine, Qilu Hospital, Shandong University, Wenhua Xi Road 107, Jinan, 250012, Shandong, China

    Yingjie Zhao

  3. Department of Infectious Diseases, Affiliated Hospital of Qingdao University, Jiangsu Road 16, Qingdao, 266000, Shandong, China

    Deyu Huang

  4. Department of Clinical Laboratory, Affiliated Hospital of Qingdao University, Jiangsu Road 16, Qingdao, 266000, Shandong, China

    Hong He

  5. Department of Infectious Diseases, Cheeloo College of Medicine, Qilu Hospital, Shandong University, Wenhua Xi Road 107, Jinan, 250012, Shandong, China

    Gang Wang & Lintao Sai

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  1. Chengxi Sun
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  2. Yingjie Zhao
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Correspondence to Lintao Sai.

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Handling Editor: Reimar Johne.

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The nucleotide sequences reported in this study had been deposited in the GenBank database under the accession numbers MT825249-MT825550.

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Sun, C., Zhao, Y., Wang, G. et al. Molecular epidemiology of GII noroviruses in outpatients with acute gastroenteritis in Shandong Province, China. Arch Virol 166, 375–387 (2021). https://doi.org/10.1007/s00705-020-04883-1

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  • DOI: https://doi.org/10.1007/s00705-020-04883-1

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