Abstract
A new virus resembling members in the genus Carlavirus was identified in an Out of Yesteryear rose (Rosa sp.) by high-throughput sequencing. The virus was discovered during the screening of a rose virus collection belonging to Foundation Plant Services (UC-Davis). The full genome of the virus is 8825 nt long, excluding a poly(A) tail, and includes six predicted genes coding for replicase, triple gene block, coat protein (CP), and nucleic acid binding protein. The closest relative of the putative virus is rose virus A (RVA; genus Carlavirus), with 75% and 78% aa sequence identity in the replicase and CP, respectively. The relationship with RVA and other carlaviruses was supported by phylogenetic analyses using replicase and CP sequences. Based on genome organization, sequence identity, and phylogenetic analysis, the virus found in the Out of Yesteryear plant represents a new member of the genus Carlavirus and is provisionally named “rose virus B” (RVB). Further testing by reverse transcription PCR confirmed the presence of RVB in the original source and seven additional rose selections from the same collection.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Diaz-Lara A, Mollov D, Golino D, Al Rwahnih M (2019) Complete genome sequence of rose virus A, the first carlavirus identified in rose. Arch Virol. https://doi.org/10.1007/s00705-019-04460-1
Diaz-Lara A, Navarro B, Di Serio F et al (2019) Two novel negative-sense RNA viruses infecting grapevine are members of a newly proposed genus within the family Phenuiviridae. Viruses 11:685. https://doi.org/10.3390/v11080685
Bankevich A, Nurk S, Antipov D et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477. https://doi.org/10.1089/cmb.2012.0021
Tatusova TA, Madden TL (1999) BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174:247–250. https://doi.org/10.1111/j.1574-6968.1999.tb13575.x
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
King AM, Lefkowitz E, Adams MJ, Carstens EB (2011) Virus taxonomy: ninth report of the international committee on taxonomy of viruses. Elsevier, Amsterdam
Al Rwahnih M, Alabi OJ, Hwang MS et al (2019) Identification and genomic characterization of grapevine Kizil Sapak virus, a novel grapevine-infecting member of the family Betaflexiviridae. Arch Virol 164:3145–3149. https://doi.org/10.1007/s00705-019-04434-3
Acknowledgements
The authors are grateful to Joshua Kohl (Foundation Plant Services) for technical assistance.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
Additional information
Handling Editor: Sead Sabanadzovic.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Diaz-Lara, A., Mollov, D., Golino, D. et al. Detection and characterization of a second carlavirus in Rosa sp.. Arch Virol 166, 321–323 (2021). https://doi.org/10.1007/s00705-020-04864-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-020-04864-4