Abstract
The leaf beetle Aulacophora lewisii (family Chrysomelidae, order Coleoptera) is a common insect pest of cucurbitaceous vegetables. In this study, the complete genome sequence of a novel virus from a single leaf beetle was determined using metagenomic sequencing and rapid amplification of cDNA ends. A homology search and phylogenetic analysis suggested that the new virus belongs to the genus Iflavirus, family Iflaviridae, and it was tentatively named “Aulacophora lewisii iflavirus 1” (ALIV1). ALIV1 has a single positive-stranded RNA genome of 9655 nucleotides in length (excluding the polyA tail) that is predicted to encode typical conserved domains of iflaviruses, including two picornavirus-like capsid protein domains, a helicase domain, and an RNA-dependent RNA polymerase (RdRp) domain. Sequence comparisons showed that the full genome sequence of ALIV1 is most similar to that of Brevicoryne brassicae picorna-like virus, with 42.4% sequence identity, and it shares 60% sequence identity in the coat protein region with its closest homolog, Watson virus. The average coverage of the ALIV1 sequence was approximately 5000X, suggesting that it might actively replicate in the host. Phylogenetic analysis based on deduced amino acid sequences suggested that ALIV1 is closely related to Dinocampus coccinellae paralysis virus. To the best of our knowledge, ALIV1 is the first virus discovered in A. lewisii and is also the first iflavirus identified in a member of the genus Aulacophora.
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Funding
This research was supported by the China National Novel Transgenic Organisms Breeding Project (2019ZX08004-004) and the Ningbo Science and Technology Innovation 2025 Major Project (2019B10004). This work was sponsored by the K.C. Wong Magna Fund at Ningbo University.
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705_2020_4859_MOESM2_ESM.tif
Fig. S1 Maximum-likelihood phylogenetic tree base on the full genome sequences of Aulacophora lewisii iflavirus 1 (ALIV1) and the other iflaviruses. Bootstrap values are shown at each node of the tree (when > 50). Scale bars represent percent divergence. The ALIV1 isolate identified in this study is indicated by a red triangle (TIF 1554 kb)
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Ye, ZX., Li, YH., Chen, JP. et al. Complete genome analysis of a novel iflavirus from a leaf beetle, Aulacophora lewisii. Arch Virol 166, 309–312 (2021). https://doi.org/10.1007/s00705-020-04859-1
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DOI: https://doi.org/10.1007/s00705-020-04859-1