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Isolation and complete genome sequencing of the virulent phage vB_EcoS_XY3 infecting multidrug-resistant Escherichia coli

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Abstract

A virulent phage, named vB_EcoS_XY3, was isolated from hospital wastewater in Xiangyang, China. Its morphological characteristics, growth parameters, adsorption rate, and pH and temperature stability were determined. Phage vB_EcoS_XY3 was found to be able to infect Escherichia coli laboratory strains and also some multidrug-resistant E. coli strains. Its complete genome consists of 51,345 base pairs of double-stranded DNA with an average GC content of 55.24% and 85 putative protein-coding genes. Forty-four genes were annotated with known functions. These results will not only provide further insights into E. coli phages but also have implications for the development of potential biocontrol agents.

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Acknowledgements

This investigation was supported by the Natural Science Foundation of Hubei Province of China (2020DFE025, 2019AHB068, 2018CFB701), the Scientific Research Project of Hubei Province Health Committee (ZY2019F028), the Innovative Team Project (2017YHKT02) from the Institute of Medicine and Nursing at Hubei University of Medicine and the Science and Technology Development Project of Xiangyang.

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Authors and Affiliations

  1. Hubei Clinical Research Center of Parkinson’s Disease, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Xiangyang No.1 People’s Hospital, Hubei University of Medicine, Shiyan, 442000, China

    Pan Fu, Qiang Zhao, Hongxia Xu, Senmao Cai, Qianqian Xu, Xiaodong Sun & Ming Sang

  2. Department of Clinical Laboratory, Xiangyang No. 1 People’s Hospital, Hubei University of Medicine, Xiangyang, 441000, China

    Li Shi & Qin Xiong

Authors
  1. Pan Fu
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Contributions

PF, QZ and MS conceived and designed the experiments. PF, QZ, LS, QX, HX and SC performed the experiments. PF, QZ, HX, SC and QX analyzed the data. PF wrote the paper. PF, QZ, HX, SC, QX, XS and MS revised the paper. All authors read and approved the final manuscript.

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Correspondence to Ming Sang.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Fig. S1

Stability of phage vB_EcoS_XY3. (A) Thermal tolerance of the phage. (B) Tolerance of the phage to treatment at different pH values. Three independent experiments were performed, and the values represented by means. (EPS 4190 kb)

Fig. S2

Kinetics of vB_EcoS_XY3 adsorption onto host cells (A) and one-step growth curve of phage vB_EcoS_XY3 (B). Data are shown as the average of triplicate experiments, and error bars indicate the standard deviation (SD). (EPS 6453 kb)

Fig. S3

Genome structure of phage vB_EcoS_XY3. The middle ring indicates the GC content (black), and the innermost ring represents the GC skew of the vB_EcoS_XY3 genome. The predicted functions of the CDSs are indicated. (EPS 10569 kb)

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Supplementary material 6 (DOC 20 kb)

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Fu, P., Zhao, Q., Shi, L. et al. Isolation and complete genome sequencing of the virulent phage vB_EcoS_XY3 infecting multidrug-resistant Escherichia coli. Arch Virol 166, 303–307 (2021). https://doi.org/10.1007/s00705-020-04844-8

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  • DOI: https://doi.org/10.1007/s00705-020-04844-8

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