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Development of a microchip electrophoresis-based, high-throughput PCR-RFLP method to type Tax 233 variants of bovine leukemia virus in Japan

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Abstract

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis (EBL). We used microchip electrophoresis in combination with automatic image analysis to develop a novel high-throughput PCR–RFLP to type the gene sequences that encode BLV Tax 233. This method revealed that 233L-Tax is more prevalent than 233P-Tax in cattle in Japan. The proportion infected with BLV carrying the gene encoding 233L-Tax was significantly higher in Holstein cattle than in Japanese Black cattle. Holsteins infected with BLV encoding 233L-Tax had higher proviral loads than did Holsteins infected with BLV encoding 233P-Tax and Japanese Blacks infected with BLV encoding 233L-Tax or 233P-Tax. The novel method developed in this study will be a useful tool for identifying cattle harboring BLV with a higher risk of EBL and viral transmission.

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Acknowledgments

This study was partly supported by the Japan Racing and Livestock Promotion Foundation.

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Authors and Affiliations

  1. Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan

    Chihiro Ochiai, Yuzuru Katagiri & Kenji Murakami

  2. Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan

    Sota Kobayashi

  3. The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu City, Gifu, 501-1193, Japan

    Ikunori Naitoh & Kenji Murakami

  4. Graduate School of Veterinary Sciences, Iwate University, 3-18-8 Ueda, Morioka, Iwate, 020-8550, Japan

    Syuji Yoneyama, Keisuke Tomita, Leng Dongze & Kenji Murakami

  5. Department of Animal Sciences, Teikyo University of Science, 2-2-1 Senju-sakuragi, Adachi-ku, Tokyo, 120-0045, Japan

    Hirokazu Hikono

Authors
  1. Chihiro Ochiai
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  2. Yuzuru Katagiri
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  3. Sota Kobayashi
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  4. Ikunori Naitoh
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  5. Syuji Yoneyama
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  6. Keisuke Tomita
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  7. Leng Dongze
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  8. Hirokazu Hikono
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  9. Kenji Murakami
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Corresponding author

Correspondence to Kenji Murakami.

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The authors declare no competing interests.

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Ochiai, C., Katagiri, Y., Kobayashi, S. et al. Development of a microchip electrophoresis-based, high-throughput PCR-RFLP method to type Tax 233 variants of bovine leukemia virus in Japan. Arch Virol 165, 2961–2966 (2020). https://doi.org/10.1007/s00705-020-04842-w

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  • DOI: https://doi.org/10.1007/s00705-020-04842-w

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