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Complete genome analysis of PaGz-1 and PaZq-1, two novel phages belonging to the genus Pakpunavirus

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Abstract

Pseudomonas phages PaGz-1 and PaZq-1, two new phages infecting Pseudomonas aeruginosa, were isolated from fresh water in Guangdong province, China. The genomes of these two phages consist of 93,975 bp and 94,315 bp and contain 175 and 172 open reading frames (ORFs), respectively. The genome sequences of PaGz-1 and PaZq-1 share 95.8% identity with a query coverage of 94%, suggesting that these two phages belong to two different species. Based on results of nucleotide sequence alignment, gene annotation, and phylogenetic analysis, we propose PaGz-1 and PaZq-1 as representative isolates of two species in the genus Pakpunavirus within the family Myoviridae.

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Acknowledgements

We are grateful to J. Feng, Institute of Microbiology of the Chinese Academy of Sciences, for providing bacterial strains. This work was supported by the National Natural Science Foundation of China (31770111, 31971350, 31800083); the Shenzhen Science Technology and Innovation Commission (JCYJ20170413153329565, JCYJ20170818160418654, JCYJ20180302145817753, JCYJ20170818155011625, KQTD2016112915000294, KQTD2015033117210153), the Engineering Laboratory for Automated Manufacturing of Therapeutic Synthetic Microbes (Shenzhen Development and Reform Commission No. [2016]1194), and the Research Instrument Development Project of the Chinese Academy of Sciences (YJKYYQ20170063).

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Author notes

  1. Lingling Wen and Ling Chen contributed equally to this study.

Authors and Affiliations

  1. CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China

    Lingling Wen, Ling Chen, Shengjian Yuan, Haoran Zhang, Yang Tan, Yue Yu, Hui Wen, Yingfei Ma, Ting Wei & Shuqiang Huang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Lingling Wen & Shengjian Yuan

  3. College of Life Science and Technology, Jinan University, Guangzhou, 510632, China

    Linyu Tian & Tingwei Yan

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  1. Lingling Wen
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Correspondence to Ting Wei or Shuqiang Huang.

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Wen, L., Chen, L., Yuan, S. et al. Complete genome analysis of PaGz-1 and PaZq-1, two novel phages belonging to the genus Pakpunavirus. Arch Virol 165, 2393–2396 (2020). https://doi.org/10.1007/s00705-020-04745-w

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  • DOI: https://doi.org/10.1007/s00705-020-04745-w

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