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Clinical evaluation of an in-house-developed real-time RT-PCR assay for serotyping of dengue virus

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Abstract

In the present study, an in-house-developed real-time RT-PCR (rRT-PCR) for serotyping of dengue virus (DENV) was evaluated for its performance, using 612 clinical samples. Compared to the composite reference standard, the in-house-developed rRT-PCR had an overall sensitivity of 97.5% and a specificity of 100%. The assay had a sensitivity of 100%, 95.6%. 96.9% and 100% for detecting DENV-1, DENV-2, DENV-3 and DENV-4, respectively. We recommend periodic evaluation of real-time RT-PCR assays for detecting DENV serotypes with a large number of samples and the use of at least two assays that target different regions of DENV genomes.

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Authors and Affiliations

  1. Dengue/Chikungunya Group, ICMR-National Institute of Virology, Pune, 411001, Maharashtra, India

    M. B. Kakade, N. Shrivastava, J. A. Patil, D. Parashar, P. S. Shah & K. Alagarasu

Authors
  1. M. B. Kakade
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  2. N. Shrivastava
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  3. J. A. Patil
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  4. D. Parashar
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  5. P. S. Shah
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  6. K. Alagarasu
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Contributions

KA, DP and PSS conceived and designed the study. MBK, NS, and JAP performed the study. KA and MBK did the statistical analysis. KA wrote the manuscript. DP critically reviewed the manuscript, and all of the authors approved the content.

Corresponding author

Correspondence to K. Alagarasu.

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None declared.

Ethics approval

Approved by the institutional ethics committee of ICMR-NIV (NIV/IEC/2018/D-8 and NIV/IEC/Jan/2020/D-24).

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Handling Editor: Zhenhai Chen.

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Kakade, M.B., Shrivastava, N., Patil, J.A. et al. Clinical evaluation of an in-house-developed real-time RT-PCR assay for serotyping of dengue virus. Arch Virol 165, 2311–2315 (2020). https://doi.org/10.1007/s00705-020-04725-0

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  • DOI: https://doi.org/10.1007/s00705-020-04725-0

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