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Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianum

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Abstract

The complete genome sequence of a putative novel potyvirus, tentatively named “Polygonatum kingianum virus 1” (PKgV1), infecting Polygonatum kingianum in China was determined (GenBank accession no. MK427056). PKgV1 has a genome organization that is typical of potyviruses, with a single large open reading frame (nt 123–9236) that encodes a 3037-aa polyprotein that is predicted to be cleaved into 10 mature proteins by virus-encoded proteases. Nine cleavage sites and several conserved motifs were identified in PKgV1 by comparative sequence analysis. Pairwise comparisons revealed that the PKgV1 polyprotein shares 52.0-56.2% nucleotide and 49.2-52.8% amino acid sequence identity with members of the genus Potyvirus. Phylogenetic analysis indicated that PKgV1 clustered with members of the genus Potyvirus and that it is closely related to but distinct from lettuce mosaic virus (LMV, accession no. KJ161186). These results suggest that Polygonatum kingianum virus 1 (PKgV1) is a new member of the genus Potyvirus of the family Potyviridae.

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Data availability

The dataset generated during the current study is available in the GenBank database (accession no. MK427056; MT198706).

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No funding was received.

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Author notes

  1. Zeli Chen and Rex Frimpong Anane contributed equally to this study.

Authors and Affiliations

  1. Key Laboratory for Agricultural Biodiversity for Pest Management of China, Ministry of Education, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen, Zhe Wang, Linyi Yang, Lu Chen, Guosong Wen & Mingfu Zhao

  2. College of Agronomy and Biotechnology, Yunnan Agricultural University, No. 95, Jinhei Road, Panlong District, Kunming, 650201, Yunnan, China

    Zeli Chen & Guosong Wen

  3. Research and Development Center for Heath Product, College of Agronomy and Biotechnology, Yunnan Agricultural University, 32 Jiaochang Donglu, Kunming, 650201, Yunnan, China

    Zeli Chen, Zhe Wang, Linyi Yang, Lu Chen, Guosong Wen & Mingfu Zhao

  4. Laboratory of Adaptive Evolution and Evolutionary Medicine, CAS Center for Excellence in Animal Evolution and Genetics, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

  5. State Key Laboratory for Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang Donglu, Kunming, 650223, Yunnan, China

    Rex Frimpong Anane

Authors
  1. Zeli Chen
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  2. Rex Frimpong Anane
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  3. Zhe Wang
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  5. Lu Chen
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  6. Guosong Wen
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  7. Mingfu Zhao
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Correspondence to Guosong Wen or Mingfu Zhao.

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Chen, Z., Anane, R.F., Wang, Z. et al. Complete genome sequence of a novel potyvirus isolated from Polygonatum kingianum. Arch Virol 165, 2127–2131 (2020). https://doi.org/10.1007/s00705-020-04717-0

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  • DOI: https://doi.org/10.1007/s00705-020-04717-0

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