Abstract
In this study, we report the complete genome sequence of a novel luteovirus detected in almond using high-throughput sequencing. The genome of the new luteovirus comprises 5,047 nucleotides, and its genomic organization is similar to that of the recently described nectarine stem pitting associated virus (NSPaV), with only four open reading frames, encoding replication-related proteins, the coat protein (CP), and a CP readthrough protein involved in the aphid transmission of luteovirids. Phylogenic and pairwise distance analyses showed that this virus shares 79% and 57.8% amino acid identity in the P1-P2 fusion protein and the P3-P5 protein, respectively, with the most closely related luteovirus, NSPaV, suggesting that it represents a novel species, for which the name “Almond associated luteovirus 1” is proposed. To our knowledge, this is the first report of an almond-infecting luteovirus.
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Acknowledgements
The authors thank the INRAE GenoToul Platform (Toulouse, France) for HTS, and Eden Darnige for proofreading the manuscript.
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The nucleotide sequence reported in this manuscript has been deposited in GenBank under accession number MT362517.
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705_2020_4715_MOESM2_ESM.tif
Supplementary material 2 Neighbor-joining phylogenetic tree based on the complete genome sequences of luteoviruses, with potato leaf roll virus and pea enation mosaic virus-1 used as outgroups. Only bootstrap values above 70% are shown (1,000 replicates). Almond luteovirus 1 is indicated by a black dot. Accession numbers are shown in parentheses. The genera to which the various viruses belong are indicated on the right. The scale bar represents 5% nucleotide sequence divergence. (TIFF 3224 kb)
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Khalili, M., Candresse, T., Faure, C. et al. Complete genome sequence of almond luteovirus 1, a novel luteovirus infecting almond. Arch Virol 165, 2123–2126 (2020). https://doi.org/10.1007/s00705-020-04715-2
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DOI: https://doi.org/10.1007/s00705-020-04715-2