Molecular characteristics of the capsid protein VP2 gene of canine parvovirus type 2 amplified from raccoon dogs in Hebei province, China

Archives of Virology volume 165, pages2453–2459(2020)Cite this article

Abstract

Canine parvovirus type 2 (CPV-2) is currently circulating in domestic and wild animals, but our knowledge about CPV-2 infections in raccoon dogs is limited. In this study, VP2 gene sequences of CPV-2 were amplified from rectal swabs of 14 diarrhetic raccoon dogs (Nyctereutes procyonoides) in Hebei province, China, in 2016 and 2017. Phylogenetic analysis of the VP2 gene sequences revealed that most of these sequences (11 of 14) belonged to the same subclade as raccoon dog strain CPV-2/Raccoon_Dog/China/DP-1/16 isolated from Shandong province in 2016. A comparison of deduced amino acid sequences revealed presence of the substitutions S297A and S27T in 11 of those 14 sequences. I418T was observed in a minority of the sequences (4 of 14). In addition, A300D and T301I, P13S and I219V, and N419K were found in three of the sequences. This study shows that CPV-2 strains with different substitutions in their VP2 amino acid sequences were spreading among raccoon dogs in Hebei during 2016 and 2017 and suggests that further studies are needed to monitor the distribution of these strains in China.

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Acknowledgements

This work was supported by the National Key R&D Program of China (2017YFD0501600), the National Natural Science Foundation of China (NSFC) [31702275 to H.Z.], the Central Committee Guides Local Science and Technology Development Project (18246629G) and the Central Public-Interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences (1610342018017).

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Author notes

  1. Rongguang Lu, Yongle Yu, and Xiangyu Zhu contributed equally to this work and should be regarded as co-first authors.

Affiliations

  1. Key Laboratory of Special Animal Epidemic Disease of Ministry of Agriculture and Rural Affairs, Institute of Special Animals and Plants Sciences, Chinese Academy of Agricultural Sciences, No. 4899 Juye Street, Changchun, 130112, Jilin, China

    Rongguang Lu, Xiangyu Zhu, Yang Wang, Shuang Lv, Ning Shi, Xiaoyu Deng, Shizhen Lian, Bo Hu & Xijun Yan

  2. State Key Laboratory of Agrobiotechnology, Department of Biochemistry and Molecular Biology, College of Biological Sciences, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China

    Rongguang Lu, Yongle Yu, Jigui Wang & Weiquan Liu

  3. Key Laboratory of Preventive Veterinary Medicine in Hebei Province, Hebei Normal University of Science & Technology, 360 Hebei Street, Qinhuangdao, 066000, Hebei, China

    Qiumei Shi

  4. School of Life Sciences and Engineering, Foshan University, No. 33 Guang yun Road, Foshan, 528225, Guangdong, China

    Hao Liu

  5. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, No. 1 Xujiaping, Lan Zhou, 730046, Gansu, China

    Minghao Yan

  6. Institute of Quality Safety and Nutrition of Agricultural Products, Jiangsu Academy of Agricultural Sciences, No. 50 Zhongling street, Nanjing, 210014, Jiangsu, China

    Hang Zhao

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  1. Rongguang Lu
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Correspondence to Weiquan Liu or Xijun Yan.

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Lu, R., Yu, Y., Zhu, X. et al. Molecular characteristics of the capsid protein VP2 gene of canine parvovirus type 2 amplified from raccoon dogs in Hebei province, China. Arch Virol 165, 2453–2459 (2020). https://doi.org/10.1007/s00705-020-04714-3

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