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Identification of proteins associated with two diverse Caulobacter phicbkvirus particles

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Abstract

Genomic evolution among bacteriophages infecting Caulobacter crescentus is inevitable. However, the conservation of the proteins associated with intact phage particles has not been investigated. In this study, we compared the structural proteins associated with two genomically diverse but morphologically similar C. crescentus-infecting bacteriophages, phiCbK and CcrSC. We were able to detect more than 20 proteins that are part of the bacteriophage particle in both phages, and we were able to identify a small number of proteins that were found in only one of the two phage particles. All but one of the genes coding for these structural proteins were located in a region of the genome that had been designated a structural region, confirming the idea that the genes in these phage genomes are clustered according to their function. During the purification process, we also discovered that phiCbk has a replication complex that can be recovered from the cell lysate, and this complex allowed us to identify many of the phage proteins involved in phage genome replication.

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Funding

This work was funded in part by National Institutes of Health grant GM076277 to BE.

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Author notes

  1. Kiesha Wilson

    Present address: Pathology, Microbiology, and Immunology Department, University of South Carolina School of Medicine, Columbia, SC, 29208, USA

Authors and Affiliations

  1. Department of Biological Sciences, University of South Carolina, Columbia, SC, 29208, USA

    Kiesha Wilson & Bert Ely

  2. Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL, 32610, USA

    Fanchao Zhu, Ran Zheng & Sixue Chen

  3. Department of Biology, Genetics Institute, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32610, USA

    Sixue Chen

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  1. Kiesha Wilson
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  2. Fanchao Zhu
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  3. Ran Zheng
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  5. Bert Ely
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Correspondence to Bert Ely.

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Wilson, K., Zhu, F., Zheng, R. et al. Identification of proteins associated with two diverse Caulobacter phicbkvirus particles. Arch Virol 165, 1995–2002 (2020). https://doi.org/10.1007/s00705-020-04707-2

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  • DOI: https://doi.org/10.1007/s00705-020-04707-2

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