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Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani

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Abstract

A double-stranded RNA (dsRNA) segment was identified in Rhizoctonia solani anastomosis group (AG)-2-2IIIB, the primary causal agent of Rhizoctonia crown and root rot of sugar beet. The dsRNA segment represented the genome replication intermediate of a new mitovirus that was tentatively designated as "Rhizoctonia solani mitovirus 39" (RsMV-39). The complete sequence of the dsRNA was 2805 bp in length with 61.9% A+U content. Using either the fungal mitochondrial or universal genetic code, a protein of 840 amino acids containing an RNA-dependent RNA polymerase (RdRp) domain was predicted with a molecular mass of 94.46 kDa. BLASTp analysis revealed that the RdRp domain of RsMV-39 had 43.55% to 72.96% sequence identity to viruses in the genus Mitovirus, and was the most similar (72.96% identical) to that of Ceratobasidium mitovirus A (CbMV-A). Phylogenetic analysis based on RdRp domains clearly showed that RsMV-39 is a member of a distinct species in the genus Mitovirus of the family Mitoviridae. This is the first full genome sequence of a mycovirus associated with R. solani AG-2-2IIIB.

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Funding

This work was financially supported by the Earmarked Fund for China Agriculture Research System (CARS-170304) and the National Training Program of Innovation and Entrepreneurship for Undergraduates (201910019031).

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Correspondence to Can Zhao or Xuehong Wu.

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Li, S., Li, Y., Hu, C. et al. Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani. Arch Virol 165, 1719–1723 (2020). https://doi.org/10.1007/s00705-020-04664-w

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  • DOI: https://doi.org/10.1007/s00705-020-04664-w

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