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Whole-genome sequence of a reassortant G9P[4] rotavirus A strain from two children in the Czech Republic

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Abstract

An unusual reassortant rotavirus A (RVA) strain was isolated during RVA surveillance in two previously hospitalized children in 2018. G/P typing revealed uncommon G9P[4] genotypes, so the strains were further characterized by Illumina next-generation sequencing. Whole-genome typing showed that the two strains had a DS-1-like backbone except for NSP2: G9-P[4]-I2-R2-C2-M2-A2-N1-T2-E2-H2. The two strains shared 99.9-100% nucleotide sequence identity in all genes.

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Funding

This work was funded by the Czech Health Research Council, grant number 16-29937A, and by the Ministry of Agriculture of the Czech Republic, Grant number RO0518.

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Authors and Affiliations

  1. Veterinary Research Institute, Brno, Czech Republic

    Romana Moutelíková & Jana Prodělalová

  2. Department of Microbiology, University Hospital Olomouc and Faculty of Medicine, Palacký University, Olomouc, Czech Republic

    Pavel Sauer

Authors
  1. Romana Moutelíková
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  2. Pavel Sauer
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  3. Jana Prodělalová
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Corresponding author

Correspondence to Romana Moutelíková.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the ethical committee of the University Hospital Olomouc, Czech Republic, and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Handling Editor: Reimar Johne.

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The GenBank accession numbers for the sequences of the two human RVA strains reported in this paper are MT005287-MT005308.

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Moutelíková, R., Sauer, P. & Prodělalová, J. Whole-genome sequence of a reassortant G9P[4] rotavirus A strain from two children in the Czech Republic. Arch Virol 165, 1703–1706 (2020). https://doi.org/10.1007/s00705-020-04648-w

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  • DOI: https://doi.org/10.1007/s00705-020-04648-w

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