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Genomic characterization of cycad leaf necrosis virus, the first badnavirus identified in a gymnosperm

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Abstract

A previously undescribed badnavirus was isolated from Zamia fischeri showing symptoms of chlorosis, necrosis, and ringspot. The virus has bacilliform virions 30 nm in diameter and averaging 120 nm in length. The viral genome is 9227 bp in length and contains three open reading frames characteristic of members of the genus Badnavirus. The largest open reading frame (ORF3) encodes a putative polyprotein, with predicted domains including zinc finger, aspartic protease, reverse transcriptase (RT) and RNase H. The virus is tentatively named "cycad leaf necrosis virus" (CLNV). Within the genus Badnavirus, CLNV was most closely related to sugarcane bacilliform Guadeloupe D virus (FJ439817), sharing 69% identity at the nucleotide level in the RT + RNase H region. This virus is the first badnavirus reported to infect cycads, and it has the largest genome among the currently characterized badnaviruses.

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Funding

This work was supported by the USDA National Institute of Food and Agriculture, Hatch project #1009997 and the University of Minnesota, Twin Cities. Alvarez-Quinto R.A is supported by the Republic of Ecuador through the Ecuadorean Science and Technology Secretariat (SENESCYT).

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Authors and Affiliations

  1. Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall, 1991 Upper Buford Circle, St. Paul, MN, 55108, USA

    R. A. Alvarez-Quinto & B. E. L. Lockhart

  2. The New York Botanical Garden, Bronx, NY, USA

    J. L. Fetzer

  3. Department of Plant Biology, University of Minnesota, St. Paul, MN, 55108, USA

    N. E. Olszewski

Authors
  1. R. A. Alvarez-Quinto
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  2. B. E. L. Lockhart
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  3. J. L. Fetzer
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  4. N. E. Olszewski
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Corresponding author

Correspondence to R. A. Alvarez-Quinto.

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All of the authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by Sead Sabanadzovic.

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Alvarez-Quinto, R.A., Lockhart, B.E.L., Fetzer, J.L. et al. Genomic characterization of cycad leaf necrosis virus, the first badnavirus identified in a gymnosperm. Arch Virol 165, 1671–1673 (2020). https://doi.org/10.1007/s00705-020-04630-6

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  • DOI: https://doi.org/10.1007/s00705-020-04630-6

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