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Molecular characterization of a novel mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea

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Abstract

In this study, we report the molecular characterization of a novel positive-sense single-stranded RNA (+ssRNA) mycovirus from Botryosphaeria dothidea strain G91, which we name “Botryosphaeria dothidea botourmiavirus 1” (BdBOV-1). The complete genome of BdBOV-1 is 2547 nucleotides (nt) long, contains one open reading frame (ORF) potentially encoding an RNA-dependent RNA polymerase (RdRp), and shows a close relationship to mycoviruses of the family Botourmiaviridae. Phylogenetic analysis based on the RdRp sequences confirmed that BdBOV-1 clustered together with the members of the family Botourmiaviridae. To the best of our knowledge, this is the first report of a +ssRNA mycovirus infecting B. dothidea.

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Acknowledgements

This study was financially supported by the fund of Integrated Research and Demonstration of Reduction of Fertilizer and Pesticide but Efficiency Enhancement for Pear Cultivation in the Yangtze River Basin (no. 2018YFD0201406) and the Earmarked Fund for Pear Modern Agro-Industry Technology Research System (CARS-28-15) of the Chinese Ministry of Agriculture.

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Authors and Affiliations

  1. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Mengmeng Yang, Xiaoqi Zhou, Feng Xiao, Ni Hong & Guoping Wang

  2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Mengmeng Yang, Feng Xiao, Ni Hong & Guoping Wang

  3. College of Life Science and Technology, Yangtze Normal University, Chongqing, 408000, People’s Republic of China

    Lifeng Zhai

  4. Key Lab of Plant Pathology of Hubei Province, Wuhan, 430070, Hubei, People’s Republic of China

    Mengmeng Yang & Guoping Wang

Authors
  1. Mengmeng Yang
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  2. Xiaoqi Zhou
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  3. Lifeng Zhai
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  4. Feng Xiao
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  5. Ni Hong
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  6. Guoping Wang
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Correspondence to Guoping Wang.

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Handling Editor: Ioly Kotta-Loizou.

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Yang, M., Zhou, X., Zhai, L. et al. Molecular characterization of a novel mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea. Arch Virol 165, 1667–1670 (2020). https://doi.org/10.1007/s00705-020-04629-z

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  • DOI: https://doi.org/10.1007/s00705-020-04629-z

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