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Identification of a novel bocaparvovirus in a wild squirrel in Kunming, Yunnan Province, China

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Abstract

In December 2017, a squirrel (Callosciurus phayrei) died 2 days after capture in Kunming, and its intestinal tract, heart, liver, spleen, lung, and kidney were subjected to metagenomics analysis. Reassembly and verification by reverse transcription PCR of contigs generated by next-generation sequencing yielded a 5176-nt sequence, which was designated "squirrel bocaparvovirus" (SQBOV). Phylogenetic trees based on the aa sequences of NS1, NP1, and VP1 showed that SQBOV formed an independent branch in the bocaparvovirus phylogenetic tree. The amino acid sequence identity of the NS1 of SQBOV to those of other bocaparvoviruses was below the threshold of 85% that is used to demarcate species within the genus, indicating that it should be considered a member of a new bocaparvovirus species. To our knowledge, this is the first report of a bocaparvovirus in squirrels. Our findings will enable further studies of viral diversity in rodents and of the genetic diversity and host range of bocaparvoviruses.

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Acknowledgements

We would like to thank Professor Wu Aiping at the Suzhou Institute of Systems Medicine for data analysis.

Funding

This work was supported by Grants from the National Major Research and Development Projects (Grant no. 2016YFD0500300), National Natural Science Foundation of China (Grant nos. 31660714, 81960605 and 31460669), Basic Research Projects of Yunnan Province (Grant 2019FA015), Scientific Research Fund Project of the Yunnan Provincial Department of Education (Grant no. 2018JS253) and the Yunnan Science and Technology Talents and Platform Plan (Grant no. 2018HB046). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author information

Author notes

  1. Jiali Wang, Nan Li, Zhao Li and Lin Liu contributed equally to this work.

Authors and Affiliations

  1. Yunnan Province Hospital of Infection Disease, Kunming, 650301, Yunnan, China

    Jiali Wang

  2. Yunnan Tropical and Subtropical Animal Viral Disease Laboratory, Yunnan Animal Science and Veterinary Institute, Kunming, 650224, Yunnan, China

    Nan Li, Yuwen He, Jinxin Meng & Jinglin Wang

  3. The Agricultural Technology Service Center of Qu Shui Town, Jiangcheng County, Yunnan, 665907, China

    Zhao Li

  4. Center for Systems Medicine, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Suzhou Institute of Systems Medicine, Suzhou, 215123, China

    Lin Liu

  5. Yuxi Center for Disease Control and Prevention, Yixu, 653000, Yunnan, China

    Shunxiang Li

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  1. Jiali Wang
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  3. Zhao Li
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Corresponding authors

Correspondence to Shunxiang Li or Jinglin Wang.

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The authors have declared that no competing interests exist.

Ethical approval

Authorization for the collection of mosquitoes and animals was obtained from the Institute for Yunnan Animal Science and Veterinary Institute, Kunming, China (protocol approval number 201303035).

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Handling Editor: Sheela Ramamoorthy.

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The nucleotide sequence data reported here are available in the GenBank database under accession number MN871711.

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Wang, J., Li, N., Li, Z. et al. Identification of a novel bocaparvovirus in a wild squirrel in Kunming, Yunnan Province, China. Arch Virol 165, 1469–1474 (2020). https://doi.org/10.1007/s00705-020-04613-7

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  • DOI: https://doi.org/10.1007/s00705-020-04613-7

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