Abstract
In this study, the complete genomic sequence of a novel virus was determined by next-generation sequencing of a sample from a symptomatic strawberry plant with severe yellow spots and mosaic on its leaves. Its genomic organization and sequence showed that this virus is related to members of the proposed insect-specific genus “Negevirus”. The sample also contained sequences from the geranium aphid Acyrthosiphon malvae. Although the virus was detected repeatedly in the same plant during the three following years, no other positive samples were obtained from the surroundings or more-distant locations. Reverse transcription qPCR analysis revealed the presence of both genomic positive and complementary negative strands of the viral genome in the sample, with a 3- to 30-fold excess of the positive strand, indicating active viral replication. As the virus was not detected in any insect species collected at this location, the virus was provisionally named “Fragaria vesca-associated virus 1” (FVaV-1).
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References
Fránová J, Přibylová J, Koloniuk I (2019) Molecular and biological characterization of a new strawberry cytorhabdovirus. Viruses 11:982
Vasilakis N, Forrester NL, Palacios G et al (2013) Negevirus: a proposed new taxon of insect-specific viruses with wide geographic distribution. J Virol 87(5):2475–2488
Jiwaji M, Matcher GF, de Bruyn M-M et al (2019) Providence virus: an animal virus that replicates in plants or a plant virus that infects and replicates in animal cells? PLoS One 14(6):e0217494
Price MN, Dehal PS, Arkin AP (2010) FastTree 2—approximately maximum-likelihood trees for large alignments. PLoS One 5(3):e9490
Letunic I, Bork P (2016) Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Res 44:W242–W245
Acknowledgements
This research was supported by the Ministry of Agriculture of the Czech Republic, projects no. QK1920245 and QJ1610365, and institutional support (RVO60077344) from the Czech Academy of Sciences. We thank Jiří Houska for his technical assistance.
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Lenz, O., Přibylová, J., Fránová, J. et al. Fragaria vesca-associated virus 1: a new virus related to negeviruses. Arch Virol 165, 1249–1252 (2020). https://doi.org/10.1007/s00705-020-04603-9
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DOI: https://doi.org/10.1007/s00705-020-04603-9