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A novel narnavirus from the plant-pathogenic fungus Magnaporthe oryzae

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Abstract

A novel mycovirus with the proposed name “Magnaporthe oryzae narnavirus virus 1” (MoNV1), was described in the rice blast fungus Magnaporthe oryzae. The virus has a single-stranded (+ss) RNA genome of 2452 nucleotides, contains a single open reading frame (ORF) predicted to encode an RNA-dependent RNA polymerase (RDRP), and is closely related to some viruses of the genus Narnavirus, family Narnaviridae, including Aspergillus fumigatus narnavirus 1 (AfNV1), Neofusicoccum parvum narnavirus 2 (NpNV2) and Alternaria tenuissima narnavirus 1 (AtNV2). Genome sequence comparisons and phylogenetic analysis suggested that MoNV1 is a new member of the genus Narnavirus. The RDRPs of MoNV1 and some closely related narnaviruses do not contain a typical metal-binding “GDD” motif and catalytic site. Further studies are needed to investigate the replication mechanism of these viruses.

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References

  1. Ai Y, Zhong J, Chen C, Zhu H, Gao B (2016) A novel single-stranded rna virus isolated from the rice-pathogenic fungus magnaporthe oryzae, with similarity to members of the family tombusviridae. Arch Virol 161:725–729

    Article  CAS  PubMed  Google Scholar 

  2. Du YN, He X, Zhou X, Fang SG, Deng QC (2016) Complete nucleotide sequence of magnaporthe oryzae partitivirus 1. Arch Virol 161(11):3295–3298

    Article  CAS  PubMed  Google Scholar 

  3. Ghabrial SA, Suzuki N (2009) Viruses of plant pathogenic fungi. Annu Rev Phytopathol 47:353–384

    Article  CAS  PubMed  Google Scholar 

  4. Higashiura T, Katoh Y, Urayama S, Hayashi O, Aihara M, Fukuhara T, Fuji S, Kobayashi T, Hase S, ArieTohru T, Teraoka T, Komatsu K, Moriyama H (2019) Magnaporthe oryzae chrysovirus 1 strain D confers growth inhibition to the host fungus and exhibits multiform viral structural proteins. Virology 535:241–254

    Article  CAS  PubMed  Google Scholar 

  5. Hong Y, Dover SL, Cole TE, Brasier CM, Buck KW (1999) Multiple mitochondrial viruses in an isolate of the Dutch Elm disease fungus Ophiostoma novo-ulmi. Virology 258:118–127

    Article  CAS  PubMed  Google Scholar 

  6. Illana A, Marconi M, Rodríguez-Romero J, Xu P, Dalmay T, Wilkinson MD, Sesma A (2017) Molecular characterization of a novel ssRNA ourmia-like virus from the rice blast fungus Magnaporthe oryzae. Arch Virol 162:891–895

    Article  CAS  PubMed  Google Scholar 

  7. Kamer G, Argos P (1984) Primary structural comparison of RNA-dependent RNA polymerase from plant, animal and bacterial viruses. Nucleic Acid Res 12(18):7269–7282

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Koonin EV, Choi GH, Nuss DL, Shapira R, Carrington JC (1991) Evidence for common ancestry of a chestnut blight hypovirulenceassociated double–stranded RNA and a group of positive–strand RNA plant viruses. Proc Natl Acad Sci USA 88:10647–10651

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306

    Article  CAS  PubMed  Google Scholar 

  10. Li C, Zhu J, Gao B, Zhu H, Zhou Q, Zhong J (2019) Characterization of a novel ourmia-like mycovirus infecting Magnaporthe oryzae and implications for viral diversity and evolution. Viruses 11(3):223

    Article  CAS  PubMed Central  Google Scholar 

  11. Liu LJ, Xie JT, Cheng JS, Fu YP, Li GQ, Yi XH, Jiang DH (2014) Fungal negative-stranded rna virus that is related to bornavirus and nyavirus. Proc Natl Acad Sci USA 111(33):12205–12210

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol 59(3):307–321

    Article  CAS  PubMed  Google Scholar 

  13. Maejima K, Himeno M, Komatsu K, Kakizawa S, Yamaji Y, Hamamoto H, Namba S (2008) Complete nucleotide sequence of a new double-stranded RNA virus from the rice blast fungus, Magnaporthe oryzae. Arch Virol 153(2):389–391

    Article  CAS  PubMed  Google Scholar 

  14. Morris TJ, Dodds JA (1979) Isolation and analysis of doublestranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  15. Nerva L, Turina M, Zanzotto A, Gardiman M, Chitarra W (2019) Isolation, molecular characterization and virome analysis of culturable wood fungal endophytes in esca symptomatic and asymptomatic grapevine plants. Environ Microbiol 21(8):2886–2904

    Article  CAS  PubMed  Google Scholar 

  16. Ng KK, Arnold JJ, Cameron CE (2008) Structure-function relationships among rna-dependent rna polymerases. Curr Top Microbiol Immunol 320(1):137–156

    CAS  PubMed  PubMed Central  Google Scholar 

  17. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal–plant interface. Nat Rev Microbiol 3(8):632–642

    Article  CAS  PubMed  Google Scholar 

  18. Osaki H, Sasak A, Nomiyama K, Tomioka K (2016) Multiple virus infection in a single strain of Fusarium poae shown by deep sequencing. Virus Genes 52:835–847

    Article  CAS  PubMed  Google Scholar 

  19. Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10(1):115–128

    Article  CAS  PubMed  Google Scholar 

  20. Poch O, Sanvnget I, Delarue M, Tordo N (1989) Identification of four conserved motifs among the RNA-dependent polymerases encoding elements. EMBO J 8(12):3867–3874

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Steitz TA (1998) Structural biology: a mechanism for all polymerases. Nature 391(6664):231–232

    Article  CAS  PubMed  Google Scholar 

  22. Tang LH, Hu YP, Liu LJ, Wu SS, Xie J, Cheng JS, Fu YP, Zhang GM, Ma JT, Wang YL, Zhang LY (2015) Genomic organization of a novel victorivirus from the rice blast fungus Magnaporthe oryzae. Arch Virol 160(11):1–4

    Article  Google Scholar 

  23. Urayama S, Kato S, Suzuki Y, Aoki N, Le MT, Arie T, Teraoka T, Fukuhara T, Moriyama H (2010) Mycoviruses related to chrysovirus affect vegetative growth in the rice blast fungus Magnaporthe oryzae. J Gen Virol 91(Pt 12):3085–3094

    Article  CAS  PubMed  Google Scholar 

  24. Urayama S, Sakoda H, Takai R, Katoh Y, Le Minh T, Fukuhara T, Arie T, Teraoka T, Moriyama H (2014) A dsRNA mycovirus, Magnaporthe oryzae chrysovirus 1-B, suppresses vegetative growth and development of the rice blast fungus. Virology 448(2):265–273

    Article  CAS  PubMed  Google Scholar 

  25. Xie JT, Jiang DH (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    Article  CAS  PubMed  Google Scholar 

  26. Yokoi T, Yamashita S, Hibi T (2007) The nucleotide sequence and genome organization of Magnaporthe oryzae virus 1. Arch Virol 152(12):2265–2269

    Article  CAS  PubMed  Google Scholar 

  27. Yu X, Li B, Fu YP, Jiang DH, Yi XH (2010) A geminivirus-related dna mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107(18):8387–8392

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Zool J, Verweij PE, Melchers WJG, Ulrich M (2018) Discovery and characterization of novel aspergillus fumigatus mycoviruses. PLoS One 13(7):e0200511

    Article  Google Scholar 

  29. Zuker M (2003) Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31:3406–3415

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (NFSC No. 31201474 and No. 31972243).

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Author notes

  1. Yuan Lin and Jia Zhou contributed equally to this work.

Authors and Affiliations

  1. College of Agriculture, Yangtze University, Jingzhou, 434025, Hubei, China

    Yuan Lin, Jia Zhou, Xuan Zhou, Simin Shuai, Rendi Zhou, Hongliu An, Shouguo Fang, Songbai Zhang & Qingchao Deng

  2. College of Plant Protection, Hainan University, Haikou, 570228, Hainan, China

    Jia Zhou

  3. Hubei Engineering Research Center for Pest Forewarning and Management, Jingzhou, 434025, Hubei, China

    Hongliu An, Shouguo Fang, Songbai Zhang & Qingchao Deng

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  1. Yuan Lin
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Correspondence to Songbai Zhang or Qingchao Deng.

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Lin, Y., Zhou, J., Zhou, X. et al. A novel narnavirus from the plant-pathogenic fungus Magnaporthe oryzae. Arch Virol 165, 1235–1240 (2020). https://doi.org/10.1007/s00705-020-04586-7

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  • DOI: https://doi.org/10.1007/s00705-020-04586-7

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