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Viruses representing two new genomovirus species identified in citrus from Tunisia

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Abstract

Using a high-throughput sequencing approach, we identified four genomoviruses (family Genomoviridae) associated with a sweet orange (Citrus sinensis) plant collected in Tunisia. The ssDNA genomes of these genomoviruses, which were amplified, cloned and Sanger sequenced, range in size from 2156 to 2191 nt. Three of these viruses share > 99% full-genome pairwise sequence identity and are referred to as citrus Tunisia genomovirus 1 (CTNGmV-1). The CTNGmV-1 isolates share < 62% genome-wide pairwise nucleotide sequence identity with other genomoviruses and belong to the genus Gemykolovirus. The genome of the fourth virus, which was called CTNGmV-2, shares < 68% nucleotide sequence identity with other genomoviruses and belongs to the genus Gemycircularvirus. Based on the species demarcation criteria for members of the family Genomoviridae, CTNGmV-1 and -2 would each represent a new species. Although found associated with Citrus sp. plants, it is likely that these viruses infect fungi or other organisms associated with the plants.

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Acknowledgements

C.C-J was supported by a Research Internship Scholarships Abroad (BEPE) project (number 2019/08186-2) funded by São Paulo Research Foundation (FAPESP).

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Authors and Affiliations

  1. The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Arizona State University, Tempe, AZ, USA

    Camila Chabi-Jesus, Rafaela S. Fontenele, Simona Kraberger & Arvind Varsani

  2. Escola Superior de Agricultura Luiz de Queiroz/Esalq/USP, Piracicaba, SP, Brazil

    Camila Chabi-Jesus

  3. Instituto Biológico/IB, São Paulo, SP, Brazil

    Camila Chabi-Jesus, Pedro Luis Ramos-González & Juliana Freitas-Astúa

  4. Laboratory of Plant Protection, National Institute of Agronomic Research of Tunisia, Rue Hédi Karray, El Menzah, Tunisia

    Asma Najar

  5. Virology Laboratory, International Centre for Agricultural Research in the Dry Areas (ICARDA), Tunis, Tunisia

    Safaa G. Kumari

  6. International Centre for Agricultural Research in the Dry Areas (ICARDA), Beirut, Lebanon

    Safaa G. Kumari

  7. Embrapa Mandioca e Fruticultura/CNPMF, Cruz das Almas, BA, Brazil

    Juliana Freitas-Astúa

  8. Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Cape Town, South Africa

    Arvind Varsani

Authors
  1. Camila Chabi-Jesus
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  2. Asma Najar
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  3. Rafaela S. Fontenele
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  4. Safaa G. Kumari
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  5. Pedro Luis Ramos-González
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  6. Juliana Freitas-Astúa
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  7. Simona Kraberger
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  8. Arvind Varsani
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Correspondence to Asma Najar or Arvind Varsani.

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GenBank accession nos. MN708482–MN708485.

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Chabi-Jesus, C., Najar, A., Fontenele, R.S. et al. Viruses representing two new genomovirus species identified in citrus from Tunisia. Arch Virol 165, 1225–1229 (2020). https://doi.org/10.1007/s00705-020-04569-8

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  • DOI: https://doi.org/10.1007/s00705-020-04569-8

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