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Genetic diversity of group A rotaviruses in Moscow in 2018-2019

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Abstract

Here, we present the results of a study in which 639 samples obtained between October 2018 and April 2019 from patients with symptoms of acute gastroenteritis were tested for the presence of a rotavirus infection. The antigen of group A rotavirus was detected in 160 samples (25% of those tested). To study the genetic diversity of group A rotavirus, RNA was isolated from the samples, and polymerase chain reaction combined with reverse transcription (RT-PCR) with primers specific for the VP4, VP6, and VP7 genes of group A rotaviruses was performed. At least one fragment of the group A rotavirus genome was found in 101 samples (15.8%). These fragments were sequenced, and their G and P genotypes—as well as their combinations—were determined. The predominant G genotypes were G9 (35.8% of all genotyped samples) and G4 (28.4%), but the rare G12 genotype was also found (3.0%). The dominant P genotype was P[8]. The spectrum of certain G/P combinations of genotypes included seven variants. The most common variants were G9P[8] (37.2%) and G4P[8] (30.2%).

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Authors and Affiliations

  1. NextBio LLC, Moscow, Russia

    A. A. Ivashechkin

  2. N. F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Moscow, Russia

    A. G. Yuzhakov, T. V. Grebennikova, K. A. Yuzhakova, N. Y. Kulikova, L. B. Kisteneva & M. G. Almazova

  3. Moscow Clinical Hospital № 1 of Infectious Diseases, Moscow, Russia

    S. V. Smetanina & M. V. Bazarova

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  1. A. A. Ivashechkin
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Ivashechkin, A.A., Yuzhakov, A.G., Grebennikova, T.V. et al. Genetic diversity of group A rotaviruses in Moscow in 2018-2019. Arch Virol 165, 691–702 (2020). https://doi.org/10.1007/s00705-020-04534-5

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