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A novel circular Rep-encoding single-stranded DNA virus detected in Agrotis ipsilon (Lepidoptera: Noctuidae) in China

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Abstract

Long-distance migratory insects carry microorganisms that can potentially play a crucial role in the life cycles of their hosts. Here, we used Illumina and Sanger sequencing to determine the complete genome sequence of a novel circular Rep-encoding single-stranded (ss) DNA virus from an important migratory pest, Agrotis ipsilon (Hufnagel). The full genome of this new virus is about 2, 242 nt in length and shares 55-75% genome-wide pairwise sequence identity with members of the family Genomoviridae but 91% nucleotide sequence identity with finch-associated genomovirus 3 isolate S30P_D, which is tentatively abbreviated “FaGmV-3”. Viral infection rates in A. ipsilon from Yantai, Langfang and Xinxiang were 4.5% (n = 88), 11.8% (n = 85) and 0% (n = 35), respectively. Phylogenetic analysis based on the deduced amino acid sequence of Rep indicated that the Agrotis ipsilon-associated virus is closely related to members of the genus Gemykibivirus, and we propose it to be a new member of this genus. Hence, it is tentatively named “Agrotis ipsilon-associated genomovirus” (AiGmV).

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Funding

This work was supported by the National Natural Science Funds of China (Grant no. 31621064) and China Agriculture Research System (Grant no. CARS-15-19).

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Authors and Affiliations

  1. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China

    Fan Yang

  2. Fuzhou University Zhicheng College, Fuzhou, 350002, People’s Republic of China

    Fan Yang

  3. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, People’s Republic of China

    Xianming Yang & Kongming Wu

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  1. Fan Yang
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  2. Xianming Yang
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  3. Kongming Wu
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Correspondence to Kongming Wu.

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Yang, F., Yang, X. & Wu, K. A novel circular Rep-encoding single-stranded DNA virus detected in Agrotis ipsilon (Lepidoptera: Noctuidae) in China. Arch Virol 165, 771–774 (2020). https://doi.org/10.1007/s00705-019-04521-5

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  • DOI: https://doi.org/10.1007/s00705-019-04521-5

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