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Phage vB_BmeM-Goe8 infecting Bacillus megaterium DSM319


vB_BmeM-Goe8 is a phage preying on Bacillus megaterium. Its genome has a GC content of 38.9%, is 161,583 bp in size, and has defined ends consisting of 7436-bp-long terminal repeats. It harbours 11 genes encoding tRNAs and 246 coding DNA sequences, 66 of which were annotated. The particle reveals Myoviridae morphology, and the formation of a double baseplate upon tail sheath contraction indicates morphological relatedness to the group of SPO1-like phages. BLASTn comparison against the NCBI non-redundant nucleotide database revealed that Bacillus phage Mater is the closest relative of vB_BmeM-Goe8.

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  1. 1.

    Vary PS (1994) Prime time for Bacillus megaterium. Microbiology 140(Pt 5):1001–1013

  2. 2.

    Vary PS, Biedendieck R, Fuerch T, Meinhardt F, Rohde M, Deckwer W-D, Jahn D (2007) Bacillus megaterium—from simple soil bacterium to industrial protein production host. Appl Microbiol Biotechnol 76:957–967

  3. 3.

    Rygus T, Scheler A, Allmansberger R, Hillen W (1991) Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155:535–542

  4. 4.

    Liu ZL, Sinclair JB (1992) Population dynamics of Bacillus megaterium strain B153-2-2 in the rhizosphere of soybean. Phytopathology 82:1297

  5. 5.

    Lancaster JC, Hodde MK, Hernandez AC, Kuty Everett GF (2015) Complete genome sequence of Bacillus megaterium myophage mater. Genome Announc 3(1):e01424-14

  6. 6.

    Cadungog JN, Khatemi BE, Hernandez AC, Kuty Everett GF (2015) Complete genome sequence of Bacillus megaterium myophage moonbeam. Genome Announc 3(1):e01428-14

  7. 7.

    Klumpp J, Lavigne R, Loessner MJ, Ackermann HW (2010) The SPO1-related bacteriophages. Arch Virol 155:1547–1561

  8. 8.

    Nordmann B, Schilling T, Hoppert M, Hertel R (2019) Complete genome sequence of the virus isolate vB_BthM-Goe5 infecting Bacillus thuringiensis. Arch Virol 164:1485–1488

  9. 9.

    Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD et al (2012) SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol 19:455–477

  10. 10.

    Willms IM, Hoppert M, Hertel R (2017) Characterization of Bacillus subtilis viruses vB_BsuM-Goe2 and vB_BsuM-Goe3. Viruses 9:146

  11. 11.

    Seemann T (2014) Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069

  12. 12.

    Jones P, Binns D, Chang H-Y, Fraser M, Li W, McAnulla C, McWilliam H, Maslen J, Mitchell A, Nuka G et al (2014) InterProScan 5: genome-scale protein function classification. Bioinformatics 30:1236–1240

  13. 13.

    Sullivan MJ, Petty NK, Beatson SA (2011) Easyfig: a genome comparison visualizer. Bioinformatics 27:1009–1010

  14. 14.

    Lechner M, Findeiß S, Steiner L, Marz M, Stadler PF, Prohaska SJ (2011) Proteinortho: detection of (co-)orthologs in large-scale analysis. BMC Bioinform 12:124

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We would like to thank Professor Dr. Rolf Daniel for scientific advice and guidance, and Dr. Anja Poehlein for sequencing. We thank Birthe Nordmann and Tobias Schilling for their help in the lab. We thank Avril von Hoyningen-Huene for proofreading the manuscript. This project was funded by the Volkswagen Foundation (Re. 94045).

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Correspondence to Robert Hertel.

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Baena Lozada, L.P., Hoppert, M. & Hertel, R. Phage vB_BmeM-Goe8 infecting Bacillus megaterium DSM319. Arch Virol 165, 515–517 (2020).

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