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First report of a pseudorabies-virus-infected wolf (Canis lupus) in China

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Abstract

We provide the first report of a wolf infected with pseudorabies virus (PRV) in China. We observed the clinical symptoms and also dissected tissue samples from the wolf. The samples were ground under sterile conditions and injected subcutaneously into the necks of rabbits, which subsequently developed intense pruritus symptoms and died. The PRV strain from the wolf was isolated in porcine kidney (PK)-15 cells and was specifically recognized by pig PRV antibody-positive serum, as shown by indirect immunofluorescence. Tissues from the dead wolf and rabbits were examined by polymerase chain reaction (PCR), and the PCR-amplified partial glycoprotein E gene was sequenced, which confirmed that the wolf had died as a result of PRV infection.

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Funding

This work was supported by the following funding: the National Natural Sciences Foundation of China (31802170), Colleges Key Scientific Research Projects of Henan Province (19B230001), Ph.D. Research Start-up Foundation of Anyang Institute of Technology (BSJ2016008).

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Authors and Affiliations

  1. School of Biotechnology and Food Science, Anyang Institute of Technology, Anyang, 455000, China

    Kaiqi Lian, Mingliang Zhang, Lingling Zhou, Yuwei Song, Guodong Wang & Shuangshan Wang

  2. Academician Workstation of Animal Disease Control and Nutrition Immunity in Henan Province, Anyang, 455000, China

    Kaiqi Lian & Shuangshan Wang

  3. Henan Joint International Research Laboratory of Veterinary Biologics Research and Application, Anyang, 455000, China

    Kaiqi Lian

Authors
  1. Kaiqi Lian
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  2. Mingliang Zhang
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  3. Lingling Zhou
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  4. Yuwei Song
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  5. Guodong Wang
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  6. Shuangshan Wang
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Corresponding author

Correspondence to Shuangshan Wang.

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The authors declare that they have no competing interests.

Ethical approval

All experiments complied with institutional animal care guidelines and were approved by the Animal Care Committee of Anyang Institute of Technology and the Animal Centre of Zhengzhou University (license no. SCXK(YU)2017-0001).

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Handling Editor: Cristina Bratanich.

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Lian, K., Zhang, M., Zhou, L. et al. First report of a pseudorabies-virus-infected wolf (Canis lupus) in China. Arch Virol 165, 459–462 (2020). https://doi.org/10.1007/s00705-019-04502-8

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  • DOI: https://doi.org/10.1007/s00705-019-04502-8

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