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TAC1, an unclassified bacteriophage of the family Myoviridae infecting Acinetobacter baumannii with a large burst size and a short latent period

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Abstract

Bacteriophage TAC1 was isolated using a clinical isolate of Acinetobacter baumannii as the host. It showed stability over wide pH and temperature range and has exhibited in vitro antibacterial activity when applied at an MOI of 1. It demonstrated a broad intraspecies host range and infected 66% of the isolates tested. It has produced 454 virions from a single bacterium with a short latent period of 15 minutes. TAC1 has a linear dsDNA genome with a length of 101.77 kb and 37.5% GC content. The genome encodes 161 proteins and 13 putative tRNAs. Whole-genome sequence comparisons using BLASTn and phylogenetic analysis showed that TAC1 is related to unclassified bacteriophages of the family Myoviridae.

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Data availability

The genome sequence has been submitted to the NCBI GenBank database (accession no. MK170160).

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Acknowledgements

We acknowledge Cody S. Sheik, Swenson College of Science and Engineering, University of Minnesota Duluth, USA, for helping us with genome sequencing. We are also very thankful to the Higher Education Commission (HEC) of Pakistan for providing funds (HEC-NRPU-4501) to conduct this research.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan

    Muhammad Asif, Iqbal Ahmad Alvi, Rabia Tabassum & Shafiq Ur Rehman

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  1. Muhammad Asif
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  2. Iqbal Ahmad Alvi
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  3. Rabia Tabassum
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  4. Shafiq Ur Rehman
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Contributions

M.A. conducted the majority of the experiments, and Dr. S.R. supervised all experiments and the manuscript write-up. I.A.A. performed the genome analysis, and R.T. helped in characterizing the TAC1 bacteriophage. All authors have reviewed the final version of the manuscript.

Corresponding author

Correspondence to Shafiq Ur Rehman.

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The authors declare no competing interests.

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Handling Editor: Chan-Shing Lin.

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Asif, M., Alvi, I.A., Tabassum, R. et al. TAC1, an unclassified bacteriophage of the family Myoviridae infecting Acinetobacter baumannii with a large burst size and a short latent period. Arch Virol 165, 419–424 (2020). https://doi.org/10.1007/s00705-019-04483-8

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  • DOI: https://doi.org/10.1007/s00705-019-04483-8

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