Skip to main content
SpringerLink
Log in

Complete genome sequence of a novel capillovirus infecting Hevea brasiliensis in China

  • Annotated Sequence Record
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Tapping panel dryness (TPD) is a complex disorder that causes partial or complete cessation of latex drainage upon tapping of rubber trees (Hevea brasiliensis). In this work, we determined the complete genome sequences of a novel virus identified in a rubber tree with TPD syndrome in China. The genome of the virus consists of 6811 nt and possesses two overlapping open reading frames (ORF1 and ORF2), encoding a polyprotein and a movement protein, respectively. The polyprotein shares 37% amino acid sequence identity with cherry virus A (CVA, ARQ83874.1) over 99% coverage. The genome architecture is similar to that of members of the genus Capillovirus (family Betaflexiviridae). Phylogenetic analysis of the replicase proteins showed that the virus clustered together with members of the genus Capillovirus. The new virus is tentatively called “rubber tree virus 1” (RTV1). RTV1 is the first virus reported to infect rubber trees. This work lays a foundation for research into finding the potential causal agent of TPD in Hevea brasiliensis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Chen S, Peng S, Huang G, Wu K, Fu X, Chen Z (2003) Association of decreased expression of a Myb transcription factor with the TPD (tapping panel dryness) syndrome in Hevea brasiliensis. Plant Mol Biol 51(1):51–58

    Article  CAS  Google Scholar 

  2. Venkatachalam P, Thulaseedharan A, Raghothama K (2007) Identification of expression profiles of tapping panel dryness (TPD) associated genes from the latex of rubber tree (Hevea brasiliensis Muell. Arg.). Planta 226(2):499–515

    Article  CAS  Google Scholar 

  3. de Faÿ E, Jacob JL (1989) Anatomical organization of the laticiferous system in the bark of Hevea brasiliensis. In: Auzac JD, Jacob L, Chrestin H (eds) Physiology of the rubber tree latex. CRC Press, Boca Raton, pp 3–14

    Google Scholar 

  4. Kumar A, Pandey DM, Abraham T, Mathew J, Ramachandran P, Malathi VG (2013) Determination of biotic aetiology of tapping panel dryness (TPD) syndrome of rubber tree (Hevea brasiliensis) by return-polyacrylamide gel electrophoresis (R-PAGE) technique. Arch Phytopath Plant Prot 46(6):710–720

    Article  CAS  Google Scholar 

  5. Pellegrin F, Nandris D, Chrestin H, Duran-Vila N (2004) Rubber tree (Hevea brasiliensis) bark necrosis syndrome I: still no evidence of a biotic causal agent. Plant Dis 88(9):1046

    Article  CAS  Google Scholar 

  6. Adams MJ, Candresse T, Hammond J, Kreuze JF, Martelli GP, Namba S, Pearson MN, Ryu KH, Saldarelli P, Yoshikawa N (2012) Family—Betaflexiviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy. Ninth report of the International Committee on Taxonomy of Viruses, pp 920–941

  7. Rubino L, Stradis AD, Martelli GP (2012) Tepovirus, a novel genus in the family Betaflexiviridae. Arch Virol 157:1629–1633

    Article  CAS  Google Scholar 

  8. Adams MJ, Antoniw JF, Bar-Joseph M, Brunt AA, Candresse T, Foster GD, Martelli GP, Milne RG, Zavriev SK, Fauquet CM (2004) The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149(5):1045–1060

    Article  CAS  Google Scholar 

  9. Martelli GP, Adams MJ, Kreuze JF, Dolja VV (2007) Family Flexiviridae: a case study in virion and genome plasticity. Ann Rev Phytopath 45:73–100

    Article  CAS  Google Scholar 

  10. Jo Y, Choi H, Kim SM, Kim SL, Lee BC, Cho WK (2016) Integrated analyses using RNA-Seq data reveal viral genomes, single nucleotide variations, the phylogenetic relationship, and recombination for apple stem grooving virus. BMC Genom 17:579

    Article  Google Scholar 

  11. Magome H, Yoshikawa N, Takahashi T, Ito T, Miyakawa T (1997) Molecular variability of the genomes of capilloviruses from apple, Japanese pear, European pear, and citrus trees. Phytopathology 87(4):389–396

    Article  CAS  Google Scholar 

  12. Kesanakurti P, Belton M, Saeed H, Rast H, Boyes I, Rott M (2017) Comparative analysis of cherry virus A genome sequences assembled from deep sequencing data. Arch Virol 162(9):2821–2828

    Article  CAS  Google Scholar 

  13. Petrzik K, Pribylova J, Koloniuk I, Spak J (2016) Molecular characterization of a novel capillovirus from red currant. Arch Virol 161(4):1083–1086

    Article  CAS  Google Scholar 

  14. Shim H, Min Y, Hong S, Kwon M, Kim D, Kim H, Choi Y, Lee S, Yang J (2004) Nucleotide sequences of a Korean isolate of apple stem grooving virus associated with black necrotic leaf spot disease on pear (Pyrus pyrifolia). Mol Cells 18(2):192–199

    CAS  PubMed  Google Scholar 

  15. Komatsu K, Hirata H, Fukagawa T, Yamaji Y, Okano Y, Ishikawa K, Adachi T, Maejima K, Hashimoto M, Namba S (2012) Infection of capilloviruses requires subgenomic RNAs whose transcription is controlled by promoter-like sequences conserved among flexiviruses. Virus Res 167(1):8–15

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the Chinese National Natural Science Foundation (nos. 31860194 and 31860222).

Author information

Author notes

  1. Zhaotong Li and Hongxing Wang contributed equally to this work.

Authors and Affiliations

  1. Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Tropical Crops, Hainan University, Haikou, 570228, Hainan, People’s Republic of China

    Zhaotong Li, Hongxing Wang, Ruibai Zhao, Ze Zhang, Zhihui Xia, Jinling Zhai & Xi Huang

Authors
  1. Zhaotong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hongxing Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruibai Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ze Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhihui Xia
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jinling Zhai
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Xi Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xi Huang.

Ethics declarations

Conflict of interest

All authors declare that they have no conflicts of interest.

Ethics approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Communicated by Sead Sabanadzovic.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 119 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, Z., Wang, H., Zhao, R. et al. Complete genome sequence of a novel capillovirus infecting Hevea brasiliensis in China. Arch Virol 165, 249–252 (2020). https://doi.org/10.1007/s00705-019-04459-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-019-04459-8

Search

Navigation