Archives of Virology

, Volume 164, Issue 11, pp 2891–2894 | Cite as

Complete genome sequence of a divergent strain of potato virus P isolated from Solanum tuberosum in Russia

  • Hironobu YanagisawaEmail author
  • Yosuke Matsushita
  • Aleksandr Khiutti
  • Nina Mironenko
  • Yasuo Ohto
  • Olga Afanasenko
Annotated Sequence Record


Contigs with sequence similarity to potato virus P (PVP), which belongs to the genus Carlavirus, were identified by high-throughput sequencing analysis in potato tubers collected from a farmer’s potato production field in Surazhevka, Artyom, Primorskiy Krai (Russia) in 2018. The complete genome sequence of this virus consisted of 8,394 nucleotides, excluding the poly(A) tail. This is the first report of PVP being detected outside South America. The isolate had high sequence similarity to PVP isolates from Argentina and Brazil, but low sequence similarity was observed in the genes encoding the RNA-dependent RNA polymerase (69% nucleotide sequence identity and 80% amino acid sequence identity) and coat protein (78% nucleotide sequence identity and 89% amino acid sequence identity). Phylogenetic analysis revealed that this PVP-like virus clustered with known PVP isolates but was distinct from them. Comparison of the sequences using the classification criteria of the ICTV indicated that this PVP-like virus is a strain of PVP.



We thank Dr. T. K. Kovalenko, the Head of the Department of Biological Methods of Plant Protection at the Federal State Budget Scientific Institution “Far Eastern Research Institute of Plant Protection”, for helping us sample the potato tubers, and also Y. Narita, J. Sato, and Y. Matsumura for preparing the experimental materials. This study was supported, in part, by the “Pilot Program of International Collaborative Research (Collaborative Research with Russia in Agriculture)” under the “Commissioned Projects for Promotion of Strategic International Collaborative Research for Utilizing Advanced Technologies in Agriculture, Forestry and Fisheries” administered by the Ministry of Agriculture, Forestry and Fisheries in Japan.

Compliance with ethical standards

Conflict of interest

The authors declare no competing interests.

Ethical approval

This article does not contain any experiments involving humans or animals that have been performed by any of the authors.

Supplementary material

705_2019_4397_MOESM1_ESM.pptx (186 kb)
Supplementary material 1 (PPTX 186 kb)


  1. 1.
    Daniels J, Carvalho TCP, Bisognin DA (1993) Um novo virus latente de batata encontrado no Rio Grande do Sul. Fitopat Bras 18:287Google Scholar
  2. 2.
    Butzonitch IP, Nieto A, Truol GAM, Colavita ML (1996) Características de un nuevo carlavirus relacionado con el virus S de la papa hallado en Balcarce, Argentina V Congreso Argentino de Virología y II Encuentro de Virόlogos Latinoamericanos Tandil, ArgentinaGoogle Scholar
  3. 3.
    Massa GA, Segretin ME, Colavita M, Riero MF, Bravo-Almonacid F, Feingold S (2006) Biological and sequence data suggest that potato rough dwarf virus (PRDV) and potato virus P (PVP) are strains of the same species. Arch Virol 151:1243–1247. CrossRefPubMedGoogle Scholar
  4. 4.
    Nisbet C, Butzonitch I, Colavita M, Daniels J, Martin J, Burns R, George E, Akhond MAY, Mulholland V, Jeffries CJ (2006) Characterization of Potato rough dwarf virus and Potato virus P: distinct strains of the same viral species in the genus Carlavirus. Plant Pathol 55:803–812. CrossRefGoogle Scholar
  5. 5.
    Massa GA, Portantier M, Segretin ME, Bravo-Almonacid FF, Feingold SE (2008) Comparison of complete sequences of potato rough dwarf virus and potato virus P and their relationships to other carlaviruses. Arch Virol 153(3):1787–1789. CrossRefPubMedGoogle Scholar
  6. 6.
    Yanagisawa H, Tomita R, Katsu K, Uehara T, Astumi G, Tateda C, Kobayashi K, Sekine K-T (2016) Combined DECS analysis and next-generation sequencing enable efficient detection of novel plant RNA viruses. Viruses 8:70. CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Chiba M, Reed JC, Prokhnevsky AI, Chapman EJ, Mawassi M, Koonin EV, Carrington JC, Dolja VV (2006) Diverse suppressors of RNA silencing enhance agroinfection by a viral replicon. Virology 346:7–14. CrossRefPubMedGoogle Scholar
  8. 8.
    Herzog E, Hemmer O, Hauser S, Meyer G, Bouzoubaa S, Fritsch C (1998) Identification of genes involved in replication and movement of peanut clump virus. Virology 248:312–322. CrossRefPubMedGoogle Scholar
  9. 9.
    Morozov SY, Solovyev AG (2003) Triple gene block: modular design of amultifunctional machine for plant virus movement. J Gen Virol 84:1351–1366. CrossRefPubMedGoogle Scholar
  10. 10.
    Lee JY, Yoo BC, Rojas MR, Gomez-Ospina N, Staehelin LA, Lucas WJ (2003) Selective trafficking of non-cell-autonomous proteins mediated by NtNCAPP1. Science 299:392–396. CrossRefPubMedGoogle Scholar
  11. 11.
    Wu CH, Lee SC, Wang CW (2011) Viral protein targeting to the cortical endoplasmic reticulum is required for cell–cell spreading in plants. J Cell Biol 193(3):521–535. CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Hironobu Yanagisawa
    • 1
    Email author
  • Yosuke Matsushita
    • 2
  • Aleksandr Khiutti
    • 3
  • Nina Mironenko
    • 3
  • Yasuo Ohto
    • 1
  • Olga Afanasenko
    • 3
  1. 1.Central Region Agricultural Research CenterNational Agriculture and Food Research OrganizationTsukubaJapan
  2. 2.Institute of Vegetable and Floriculture ScienceNational Agriculture and Food Research OrganizationTsukubaJapan
  3. 3.Federal State Budget Scientific Institution “All-Russian Institute of Plant Protection” (FSBSI VIZR)Saint-PetersburgRussia

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