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The O-polysaccharide of Escherichia coli F5, which is structurally related to that of E. coli O28ab, provides cells only weak protection against bacteriophage attack

  • A. K. Golomidova
  • O. I. Naumenko
  • S. N. Senchenkova
  • Y. A. Knirel
  • A. V. LetarovEmail author
Brief Report
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Abstract

Several types of Escherichia coli O-antigens form highly effective shields protecting the bacterial cell surface and preventing bacteriophages from interacting directly with their secondary (terminal) receptors. However, it is not clear if O-antigens of various types (O-serotypes) differ in their anti-phage protection efficacy. Here, we describe a new E. coli strain, F5, which has an E. coli O28ab-related O-antigen. Although the amount of O-antigen produced by this strain is comparable to that produced by other E. coli strains we tested, it appears to give the cells significantly lower protection against phage attack than other O-antigen types, such as the O-polysaccharide of E. coli F17, which we studied earlier.

Notes

Acknowledgements

The authors thank Dr. A.S. Dmitrenok and Prof. A.S. Shashkov for help with NMR spectroscopy, and Dr. A.O. Chizhov for measuring the mass spectrum. We are also grateful to Dr. Ember Johnson-Neuroth from The Evergreen State College, USA, for the linguistic correction of the manuscript. This work was supported partially by the Russian Science Foundation Grant no. 15-15-00134P (structural analysis of the O-antigen and screening of environmental objects).

Supplementary material

705_2019_4371_MOESM1_ESM.docx (487 kb)
Supplementary material 1 (DOCX 486 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.S. N. Winogradsky Institute of Microbiology, Federal Research Centre, “Fundamentals of Biotechnology”, Russian Academy of SciencesMoscowRussian Federation
  2. 2.N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of SciencesMoscowRussian Federation
  3. 3.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussian Federation

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