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The O-polysaccharide of Escherichia coli F5, which is structurally related to that of E. coli O28ab, provides cells only weak protection against bacteriophage attack

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Abstract

Several types of Escherichia coli O-antigens form highly effective shields protecting the bacterial cell surface and preventing bacteriophages from interacting directly with their secondary (terminal) receptors. However, it is not clear if O-antigens of various types (O-serotypes) differ in their anti-phage protection efficacy. Here, we describe a new E. coli strain, F5, which has an E. coli O28ab-related O-antigen. Although the amount of O-antigen produced by this strain is comparable to that produced by other E. coli strains we tested, it appears to give the cells significantly lower protection against phage attack than other O-antigen types, such as the O-polysaccharide of E. coli F17, which we studied earlier.

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Acknowledgements

The authors thank Dr. A.S. Dmitrenok and Prof. A.S. Shashkov for help with NMR spectroscopy, and Dr. A.O. Chizhov for measuring the mass spectrum. We are also grateful to Dr. Ember Johnson-Neuroth from The Evergreen State College, USA, for the linguistic correction of the manuscript. This work was supported partially by the Russian Science Foundation Grant no. 15-15-00134P (structural analysis of the O-antigen and screening of environmental objects).

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Authors and Affiliations

  1. S. N. Winogradsky Institute of Microbiology, Federal Research Centre, “Fundamentals of Biotechnology”, Russian Academy of Sciences, 117312, Moscow, Russian Federation

    A. K. Golomidova, Y. A. Knirel & A. V. Letarov

  2. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation

    O. I. Naumenko, S. N. Senchenkova & Y. A. Knirel

  3. Faculty of Biology, Lomonosov Moscow State University, 119991, Moscow, Russian Federation

    A. V. Letarov

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  1. A. K. Golomidova
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  2. O. I. Naumenko
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  3. S. N. Senchenkova
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  4. Y. A. Knirel
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  5. A. V. Letarov
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Correspondence to A. V. Letarov.

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Golomidova, A.K., Naumenko, O.I., Senchenkova, S.N. et al. The O-polysaccharide of Escherichia coli F5, which is structurally related to that of E. coli O28ab, provides cells only weak protection against bacteriophage attack. Arch Virol 164, 2783–2787 (2019). https://doi.org/10.1007/s00705-019-04371-1

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