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Seroprevalence of porcine bocavirus in pigs in north-central China using a recombinant-NP1-protein-based indirect ELISA

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Abstract

Porcine bocavirus (PBoV), which belongs the genus Bocaparvovirus, has been identified throughout the world. However, serological methods for detecting anti-PBoV antibodies are presently limited. In the present study, an indirect enzyme-linked immunosorbent assay (PBoV-rNP1 ELISA) based on a recombinant form of nucleoprotein 1 (NP1) of PBoV was established for investigating the seroprevalence of PBoV in 2025 serum specimens collected in north-central China from 2016 to 2018, and 42.3% of the samples tested positive for anti-PBoV IgG antibodies, indicating that the seroprevalence of PBoV is high in pig populations in China.

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References

  1. Blomstrom AL, Belak S, Fossum C, Fuxler L, Wallgren P, Berg M (2010) Studies of porcine circovirus type 2, porcine boca-like virus and torque teno virus indicate the presence of multiple viral infections in postweaning multisystemic wasting syndrome pigs. Virus Res 152:59–64

    Article  CAS  PubMed  Google Scholar 

  2. Choi MG, Park SJ, Nguyen VG, Chung HC, Kim AR, Park BK (2014) Molecular detection and genetic analysis of porcine bocavirus in Korean domestic swine herds. Adv Virol 159:1487–1492

    CAS  Google Scholar 

  3. Csagola A, Lorincz M, Cadar D, Tombacz K, Biksi I, Tuboly T (2012) Detection, prevalence and analysis of emerging porcine parvovirus infections. Adv Virol 157:1003–1010

    CAS  Google Scholar 

  4. Huang J, Mor SK, Erber J, Voss E, Goyal SM (2014) Detection and characterization of porcine bocavirus in the United States. Adv Virol 159:1797–1801

    CAS  Google Scholar 

  5. Jiang YH, Xiao CT, Yin SH, Gerber PF, Halbur PG, Opriessnig T (2014) High prevalence and genetic diversity of porcine bocaviruses in pigs in the USA, and identification of multiple novel porcine bocaviruses. J Gen Virol 95:453–465

    Article  CAS  PubMed  Google Scholar 

  6. Lau SK, Woo PC, Yip CC, Li KS, Fu CT, Huang Y, Chan KH, Yuen KY (2011) Co-existence of multiple strains of two novel porcine bocaviruses in the same pig, a previously undescribed phenomenon in members of the family Parvoviridae, and evidence for inter- and intra-host genetic diversity and recombination. J Gen Virol 92:2047–2059

    Article  CAS  PubMed  Google Scholar 

  7. Li B, Xiao S, Ma J, Liu Y, Mao L, Wen L, Mao A, Zhang X, Ni Y, Guo R, Zhou J, Yu Z, Lv L, Wang X, Fang L, Chen H, He K (2011) Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV). Virol J 8:357

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Liu M, Li Y, Sun D, Xia Y, Huang J, Guo L (2014) Detection and genetic analysis of porcine bocavirus in different swine herds in North Central China. Sci World J 2014:947084

    Google Scholar 

  9. McKillen J, McNeilly F, Duffy C, McMenamy M, McNair I, Hjertner B, Millar A, McKay K, Lagan P, Adair B, Allan G (2011) Isolation in cell cultures and initial characterisation of two novel bocavirus species from swine in Northern Ireland. Vet Microbiol 152:39–45

    Article  PubMed  Google Scholar 

  10. McNair I, McNeilly F, Duffy C, McKillen J, McMenamy M, Welsh M, Allan G (2011) Production, characterisation and applications of monoclonal antibodies to two novel porcine bocaviruses from swine in Northern Ireland. Adv Virol 156:2157–2162

    CAS  Google Scholar 

  11. Sukhu L, Fasina O, Burger L, Rai A, Qiu J, Pintel DJ (2013) Characterization of the nonstructural proteins of the bocavirus minute virus of canines. J Virol 87:1098–1104

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Sun Y, Chen AY, Cheng F, Guan W, Johnson FB, Qiu J (2009) Molecular characterization of infectious clones of the minute virus of canines reveals unique features of bocaviruses. J Virol 83:3956–3967

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Xiao CT, Halbur PG, Opriessnig T (2013) Molecular evolutionary genetic analysis of emerging parvoviruses identified in pigs. Infect Genet Evol 16:369–376

    Article  CAS  PubMed  Google Scholar 

  14. Yang WZ, Yu JM, Li JS, Cheng WX, Huang CP, Duan ZJ (2012) Genome characterization of a novel porcine bocavirus. Adv Virol 157:2125–2132

    CAS  Google Scholar 

  15. Zeng S, Wang D, Fang L, Ma J, Song T, Zhang R, Chen H, Xiao S (2011) Complete coding sequences and phylogenetic analysis of porcine bocavirus. J Gen Virol 92:784–788

    Article  CAS  PubMed  Google Scholar 

  16. Zhai S, Yue C, Wei Z, Long J, Ran D, Lin T, Deng Y, Huang L, Sun L, Zheng H, Gao F, Zheng H, Chen S, Yuan S (2010) High prevalence of a novel porcine bocavirus in weanling piglets with respiratory tract symptoms in China. Adv Virol 155:1313–1317

    CAS  Google Scholar 

  17. Zhang Q, Hu R, Tang X, Wu C, He Q, Zhao Z, Chen H, Wu B (2013) Occurrence and investigation of enteric viral infections in pigs with diarrhea in China. Adv Virol 158:1631–1636

    CAS  Google Scholar 

  18. Zhang R, Fang L, Wang D, Cai K, Zhang H, Xie L, Li Y, Chen H, Xiao S (2015) Porcine bocavirus NP1 negatively regulates interferon signaling pathway by targeting the DNA-binding domain of IRF9. Virology 485:414–421

    Article  CAS  PubMed  Google Scholar 

  19. Zhang R, Fang L, Wu W, Zhao F, Song T, Xie L, Li Y, Chen H, Xiao S (2016) Porcine bocavirus NP1 protein suppresses type I IFN production by interfering with IRF3 DNA-binding activity. Virus Genes 52:797–805

    Article  CAS  PubMed  Google Scholar 

  20. Zhang W, Sano N, Kataoka M, Ami Y, Suzaki Y, Wakita T, Ikeda H, Li TC (2016) Virus-like particles of porcine bocavirus generated by recombinant baculoviruses can be applied to sero-epidemic studies. Virus Res 217:85–91

    Article  CAS  PubMed  Google Scholar 

  21. Zheng X, Liu G, Opriessnig T, Wang Z, Yang Z, Jiang Y (2016) Development and validation of a multiplex conventional PCR assay for simultaneous detection and grouping of porcine bocaviruses. J Virol Methods 236:164–169

    Article  CAS  PubMed  Google Scholar 

  22. Zhou F, Sun H, Wang Y (2014) Porcine bocavirus: achievements in the past five years. Viruses 6:4946–4960

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

Thanks to Professor Yuzhong Ma for providing the necessary equipment for protein identification, and Dr. Hongwei Yu for performing the inter-laboratory reproducibility test at Ringpu Biological Research Institute.

Funding

This research was supported by the Program of the Modern Agriculture Industry Technology System Foundation of Hebei Province (HBCT2018110207), the Science and Technology Innovation Program of Hebei Province for Graduate Students (CXZZSS2018058, CXZZBS2019095), and the Innovation Fund for Technology-Based Firms of Hebei Province, China (17C1303111001).

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Author notes

  1. Qian-Kai Shi, Jian-Lou Zhang and Jing-Hui Fan contributed equally to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Hebei Agricultural University, Lucky Street, Baoding, 071001, People’s Republic of China

    Qian-Kai Shi, Jian-Lou Zhang, Wen-Yuan Gu, Lin-Shan Hou, Guang-Fu Yuan, Shao-Jie Chen, Jing-Hui Fan & Yu-Zhu Zuo

  2. Animal Diseases Control Center of Hebei, Shijiazhuang, 050053, People’s Republic of China

    Wen-Yuan Gu

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  1. Qian-Kai Shi
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Correspondence to Jing-Hui Fan or Yu-Zhu Zuo.

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Shi, QK., Zhang, JL., Gu, WY. et al. Seroprevalence of porcine bocavirus in pigs in north-central China using a recombinant-NP1-protein-based indirect ELISA. Arch Virol 164, 2351–2354 (2019). https://doi.org/10.1007/s00705-019-04325-7

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  • DOI: https://doi.org/10.1007/s00705-019-04325-7

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