Abstract
Rodent adenoviruses are important models for human disease. In contrast to the over 70 adenovirus types isolated from humans, few rodent adenoviruses are known, despite the vast diversity of rodent species. PCR and Sanger sequencing were used to investigate adenovirus diversity in wild rodents and shrews in Cameroon. Adenovirus DNA was detected in 13.8% of animals (n = 218). All detected sequences differ from known adenovirus types by more than 10% at the amino acid level, thus indicating up to 14 novel adenovirus species. These results highlight the diversity of rodent adenoviruses, their phylogeny, and opportunities for studying alternative adenovirus rodent models.
This is a preview of subscription content, log in via an institution to check access.
References
Berk AJ (2013) Adenoviridae. In: Knipe DM, Howley PM (eds) Fields Virology, sixth edition. Wolters Kluwer Health, Lippcot Williams & Wilkins, pp 1704–1731
King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ. Virus taxonomy. Classification and nomenclature of viruses, ninth report of the international committee on taxonomy of viruse. International Union of Microbiological Societies, Virology Division, Elsevier Academic Press
Radke JR, Cook JL (2018) Human adenovirus infections. Curr Opin Infect Dis 31:251–256
Ghebremedhin B (2014) Human adenovirus: viral pathogen with increasing importance. Eur J Mirobiol Immunol (Bp.) 4:26–33
Guida JD, Fejer G, Pirofski LA, Brosnan CF, Horwitz MS (1995) Mouse adenovirus type 1 causes a fatal hemorrhagic encephalomyelitis in adult C57BL/6 but not BALB/c mice. J Virol 69:7674–7681
Spindler KR, Fang L, Moore ML, Hirsch GN, Brown CC, Kajon A (2001) SJL/J mice are highly susceptible to infection by mouse adenovirus type 1. J Virol 75:12039–12046
Kaplan C, Healing TD, Evans N, Healing L, Prior A (1980) Evidence of infection by viruses in small British field rodents. J Hyg 84:285–294
Smith AL, Singleton GR, Hansen GM, Shellam G (1993) A serologic survey for viruses and Mycoplasma pulmonis among wild house mice (Mus domesticus) in southeastern Australia. J Wildl Dis 29:219–229
Moro D, Lloyd ML, Smith AL, Shellam GR, Lawson MA (1999) Murine viruses in an island population of introduced house mice and endemic short-tailed mice in Western Australia. J Wildl Dis 35:301–310
Greenwood AG, Sanchez S (2002) Serological evidence of murine pathogens in wild grey squirrels (Sciurus carolinensis) in North Wales. Vet Rec 150:543–546
Becker SD, Bennett M, Stewart JP, Hurst JL (2007) Serological survey of virus infection among wild house mice (Mus domesticus) in the UK. Lab Anim 41:229–238
Klempa B, Krüger DH, Auste B, Stanko M, Krawczyk A, Nickel KF, Uberla K, Stang A (2009) A novel cardiotropic murine adenovirus representing a distinct species of mastadenoviruses. J Virol 83:5749–5759
Zheng XY, Qiu M, Ke XM, Guan WJ, Li JM, Huo ST, Chen SW, Zhong XS, Zhou W, Xiong YQ, Ge J, Chen Q (2016) Detection of novel adenoviruses in fecal specimens from rodents and shrews in southern China. Virus Genes 52:417–421
Abendroth B, Höper D, Ulrich RG, Larres G, Beer M (2017) A red squirrel associated adenovirus identified by a combined microarray and deep sequencing approach. Arch Virol 162:3167–3172
LeBreton M, Pike BL, Saylors KE, Diffo JL, Fair JN, Rimoin AW, Ortiz N, Djoko CF, Tamoufe U, Wolfe ND (2012) Bushmeat and infectious disease emergence. In: Aguirre A et al (eds) Conservation medicine: applied cases of ecological health. Oxford University Press, Oxford
Kingdon J (2005) The Kingdon field guide to African mammals. Bloomsbury, London
Monadjem A, Taylor PJ, Denys C, Cotterill FPD (2015) Rodents of Sub-Saharan Africa: a biogeographic and taxonomic synthesis. Walter de Gruyter GmbH & Company KG, Berlin
Wellehan JF, Johnson AJ, Harrach B, Benkö M, Pessier AP, Johnson CM, Garner MM, Childress A, Jacobson ER (2004) Detection and analysis of six lizard adenoviruses by consensus primer PCR provides further evidence of a reptilian origin for the atadenoviruses. J Virol 78:13366–13369
Townzen JS, Brower AV, Judd DD (2008) Identification of mosquito bloodmeals using mitochondrial cytochrome oxidase subunit I and cytochrome b gene sequences. Med Vet Entomol 22:386–393
Steppan SJ, Schenk JJ (2017) Musoid rodent phylogenetics: 900-species tree reveals increasing diversification rates (2017). PLos One 12:e0183070
Acknowledgements
The authors would like to thank the government of Cameroon for permission to conduct this study; the staff of Metabiota and CRESAR, who assisted in sample collection and testing; and any other involved members of the PREDICT-1 consortium (http://www.vetmed.ucdavis.edu/ohi/predict/publications/Authorship.cfm). The study was undertaken as part of the global USAID-funded Emerging Pandemic Threats (EPT) PREDICT project, which focuses on enhancing the global capacity for the detection and discovery of potentially zoonotic viruses at the human-animal interface. It was made possible primarily by the generous support of the American people through the United States Agency for International Development (USAID) Emerging Pandemic Threats PREDICT program (cooperative agreement number GHN-A-OO-09-00010-00). The contents are the responsibility of the authors and do not necessarily reflect the views of USAID or the United States Government. Some funding also came from the National Institutes of Health (NIH) Director’s Pioneer Award Program (grant number DP1-OD000370), the International Research Scientist Development Award from the NIH Fogarty International Center (K01 TW00003-1), Google.org, the Skoll foundation, the US Military HIV Research Program, and the Johns Hopkins Bloomberg School of Public Health, Center for a Livable Future.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Compliance with ethical standards
Animal capture and specimen collection were approved by the Institutional Animal Care and Use Committee (IACUC, UC Davis) and the Ministry of Forestry and Wildlife in Cameroon. All authors declare that they have no conflicts of interest.
Additional information
Handling Editor: T. K. Frey.
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Diffo, J., Ndze, V.N., Ntumvi, N.F. et al. DNA of diverse adenoviruses detected in Cameroonian rodent and shrew species. Arch Virol 164, 2359–2366 (2019). https://doi.org/10.1007/s00705-019-04323-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-019-04323-9