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Pepper yellow leaf curl Aceh virus: a novel bipartite begomovirus isolated from chili pepper, tomato, and tobacco plants in Indonesia

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Abstract

During 2017, leaf samples of chili pepper (Capsicum annuum), tomato (Solanum lycopersicum), and tobacco (Nicotiana tabacum) plants exhibiting yellowing and curling symptoms were collected from Aceh province, Indonesia. These samples were used to isolate and sequence viral genomic DNA. Six isolates with complete DNA-A and DNA-B sequences of begomovirus were obtained, all of which showed >99% sequence identity to the others. DNA-A sequences shared the highest nucleotide sequence identity (89.3%–89.7%) with monopartite pepper yellow leaf curl Indonesia virus 2 (PepYLCIV2) and the second-highest sequence identity (87.3%–87.4%) with bipartite pepper yellow leaf curl Indonesia virus (PepYLCIV). The DNA-B sequences shared the highest nucleotide sequence identity (95%–97.5%) with PepYLCIV. Results of recombination analysis indicated that the novel begomovirus was a recombinant. In accordance with the guidelines for begomovirus species demarcation, these isolates should be assigned to a new species, and we have proposed the name ‘‘pepper yellow leaf curl Aceh virus’’ (PepYLCAV) for this virus.

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Acknowledgments

We would like to thank Amirddin, Fitrizal, and Dharma Setiawan for supporting our fieldwork at Aceh. This study was supported by the Japan Society for the Promotion of Science (JSPS) and Directorate General of Resources for Science Technology and Higher Education (DG-RSTHE) under the JSPS-DG-RSTHE Joint Research Program to E. Kesumawati, S. Zakaria, and S. Koeda, and JSPS KAKENHI grant number 19H02950 to S. Koeda.

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Author notes

  1. Elly Kesumawati and Shoko Okabe contributed equally to this paper.

Authors and Affiliations

  1. Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh, 23111, Indonesia

    Elly Kesumawati & Sabaruddin Zakaria

  2. Graduate School of Agriculture, Kindai University, Nara, Nara, 631-8505, Japan

    Shoko Okabe, Kanami Homma, Ikuya Fujiwara, Shinya Kanzaki & Sota Koeda

Authors
  1. Elly Kesumawati
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  2. Shoko Okabe
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  3. Kanami Homma
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  4. Ikuya Fujiwara
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  5. Sabaruddin Zakaria
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  6. Shinya Kanzaki
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  7. Sota Koeda
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Correspondence to Sota Koeda.

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Kesumawati, E., Okabe, S., Homma, K. et al. Pepper yellow leaf curl Aceh virus: a novel bipartite begomovirus isolated from chili pepper, tomato, and tobacco plants in Indonesia. Arch Virol 164, 2379–2383 (2019). https://doi.org/10.1007/s00705-019-04316-8

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  • DOI: https://doi.org/10.1007/s00705-019-04316-8

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