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Identification and genetic characterization of a porcine hepe-astrovirus (bastrovirus) in the United States

Archives of Virology volume 164, pages 2321–2326 (2019)Cite this article

Abstract

Here we describe the identification and genetic characterization of a porcine hepe-astrovirus, or bastrovirus, obtained from feces from pigs in the United States. The genome of the new bastrovirus is 5,955 nt long and contains two open reading frames (ORFs). ORF1 encodes a protein containing three domains, viral methyltransferase, RNA helicase and RNA-dependent RNA polymerase (RdRp), and is closely related to the RdRp of hepatitis E virus. The ORF2 protein shares similarities with the astrovirus capsid precursor protein. Although structural features of bastroviruses may resemble those of astroviruses, distinct characteristics of the newly identified bastrovirus include the presence of an RNA helicase domain in ORF1 and the lack of ORF1b. In addition to genetic characterization, screening of 368 porcine samples (oral fluids, oral swabs or fecal swabs) collected in the United States (US) using a porcine-bastrovirus-specific real-time PCR assay revealed that 31% of those samples were positive. These results suggest a broad distribution of bastroviruses in the swine population in the US. This represents the first description of bastrovirus in swine in the US.

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Funding

This study was funded by the USDA National Institute of Food and Agriculture (Grants SD00H517-14 and SD00R518-14).

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Authors and Affiliations

  1. Animal Disease Research and Diagnostic Laboratory (ADRDL), Department of Veterinary and Biomedical Sciences, South Dakota State University, Box 2175, 1155 N Campus Drive, SAR118, Brookings, SD, 57007, USA

    Fernando V. Bauermann, Alaine R. Buysse, Lok R. Joshi & Diego G. Diel

  2. Department of Veterinary Pathobiology, Oklahoma State University, 250 McElroy Hall, Stillwater, OK, 74074, USA

    Fernando V. Bauermann

  3. Cambridge Technologies, 1525 Bioscience Drive, Worthington, MN, 56187, USA

    Ben Hause

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  1. Fernando V. Bauermann
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  2. Ben Hause
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  4. Lok R. Joshi
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  5. Diego G. Diel
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Correspondence to Diego G. Diel.

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Bauermann, F.V., Hause, B., Buysse, A.R. et al. Identification and genetic characterization of a porcine hepe-astrovirus (bastrovirus) in the United States. Arch Virol 164, 2321–2326 (2019). https://doi.org/10.1007/s00705-019-04313-x

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  • DOI: https://doi.org/10.1007/s00705-019-04313-x