Using next-generation sequencing to characterize agents associated with a severe stunting disease of parsley from Germany, we identified a hitherto undescribed virus. We sequenced total RNA and rolling-circle-amplified DNA from diseased plants. The genome sequence of the virus shows that it is a member of the genus Nanovirus, but it lacks DNA-U4. In addition to the seven genomic DNAs of the virus, we identified a second DNA-R and seven distinct alphasatellites associated with the disease. We propose the name “parsley severe stunt associated virus” (PSSaV) for this novel nanovirus.
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The authors would like to thank Verena Maiberg and Viola Weichelt for technical assistance.
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Vetten, H.J., Knierim, D., Rakoski, M.S. et al. Identification of a novel nanovirus in parsley. Arch Virol 164, 1883–1887 (2019). https://doi.org/10.1007/s00705-019-04280-3