Archives of Virology

, Volume 164, Issue 6, pp 1609–1617 | Cite as

Characterization of a novel victorivirus isolated from the phytopathogenic fungus Botryosphaeria dothidea

  • Mengmeng Yang
  • Lifeng Zhai
  • Feng Xiao
  • Yashuang Guo
  • Min Fu
  • Ni Hong
  • Guoping WangEmail author
Original Article


Botryosphaeria dothidea is an important pathogenic fungus that causes serious diseases in fruits and trunks of many wood plant species worldwide. In this study, 28 B. dothidea strains isolated from pear trunk samples showing stem wart or canker symptoms were used to detect double-stranded RNA (dsRNA) viruses. The dsRNA bands with the size of ~ 1.0 to ~ 6.0 kbp were examined from ten strains. Here, we reported a novel dsRNA mycovirus, tentatively named as Botryosphaeria dothidea victorivirus 2 (BdVV2), isolated from the B. dothidea strain MSD53. BdVV2 contained spherical virions that were ~ 38 nm in diameter consisting of a single linear dsRNA genome of 5,090 bp in length. The BdVV2 genome contained two overlapping open reading frames (ORFs) encoding a putative coat protein (CP) and an RNA-dependent RNA polymerase (RdRp), which shared significant amino acid identities of 68% and 60% with the corresponding proteins of Sphaeropsis sapinea RNA viruses 1 (SsRV1). Phylogenetic analyses based on the aa sequences of CP and RdRp both showed that BdVV2 was phylogenetically related to the members of the genus Victorivirus in the family Totiviridae. BdVV2 is thus a novel victorivirus isolated from the phytopathogenic fungus B. dothidea.



This work was financially supported by the earmarked fund for Pear Modern Agro-Industry Technology Research System (CARS-28-15) of the Chinese Ministry of Agriculture and the Fundamental Research Funds for the Central Universities (no. 2662016PY107).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

No human or animal ethics approval was required for the completion of this study.

Informed consent

Informed consent was obtained from all individual participants included in this study.


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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  • Mengmeng Yang
    • 1
    • 2
    • 4
  • Lifeng Zhai
    • 3
  • Feng Xiao
    • 2
    • 4
  • Yashuang Guo
    • 2
    • 4
  • Min Fu
    • 2
    • 4
  • Ni Hong
    • 1
    • 2
    • 4
  • Guoping Wang
    • 1
    • 2
    • 4
    Email author
  1. 1.State Key Laboratory of Agricultural MicrobiologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  3. 3.College of Life Science and TechnologyYangtze Normal UniversityChongqingPeople’s Republic of China
  4. 4.Key Lab of Plant Pathology of Hubei ProvinceWuhanPeople’s Republic of China

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