Abstract
A nidovirus was isolated from crucian carp (Carassius auratus). The complete genome of the crucian carp nidovirus (CCNV) is 25,971 nt long and has five open reading frames, encoding the polyprotein 1ab (pp1ab), spike glycoprotein (S), membrane protein (M), and nucleocapsid protein (N). CCNV has the highest similarity to Chinook salmon nidovirus (CSNV). However, the CCNV HB93 pp1ab protein sequence has three long fragment deletions compared with the CSNV. Phylogenetic analysis based on the complete genome sequence showed that CCNV HB93 clusters with CSNV, indicating that CCNV represents a second species in the new genus Oncotshavirus within the new family Tobaniviridae in the order Nidovirales.
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Funding
This study was supported by grants from the Central-Level Non-Profit Scientific Research Institutes Special Funds (2018GH16), the Science and Technology Project of AQSIQ (2016IK116), and the National Natural Science Foundation of China (Grant 31802346).
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All animal experiments in this study were done in strict accordance with the protocols approved by the Animal Welfare Committee of China Agricultural University (permit number XK662).
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Xiao-yu, C., Yong, Z., Xin, C. et al. Isolation and genetic analysis of a nidovirus from crucian carp (Carassius auratus). Arch Virol 164, 1651–1654 (2019). https://doi.org/10.1007/s00705-019-04221-0
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DOI: https://doi.org/10.1007/s00705-019-04221-0