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Molecular characterisation of a putative new polerovirus infecting pumpkin (Cucurbita pepo) in Kenya

Archives of Virology volume 164, pages 1717–1721 (2019)Cite this article

Abstract

Next-generation sequencing of RNA extracted from a pumpkin plant with mosaic symptoms in Kenya identified the presence of a polerovirus sequence closely related to pepo aphid-borne yellows virus (PABYV). The near-complete polerovirus sequence comprised 5,810 nucleotides and contained seven putative open reading frames (ORFs) with a genome organisation typical of poleroviruses. BLASTp analysis of the translated sequences of ORFs 0, 1 and 2 revealed that their amino acid sequences differed by more than 10% from the corresponding protein sequences of other poleroviruses. These results suggest that this virus is a putative novel member of the genus Polerovirus, which has been provisionally named “pumpkin polerovirus” (PuPV).

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Acknowledgements

This project was funded by Biosciences eastern and central Africa (BecA-ILRI) Hub through the African Biosciences Challenge Fund (ABCF). DK is the recipient of an Australia Awards Scholarship.

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Authors and Affiliations

  1. Centre for Tropical Crops and Biocommodities, Queensland University of Technology, Brisbane, 4001, Australia

    Dawit B. Kidanemariam, Amit C. Sukal, James L. Dale, Robert M. Harding & Anthony P. James

  2. National Agricultural Biotechnology Research Center, Ethiopian Institute of Agricultural Research, Addis Ababa, Ethiopia

    Dawit B. Kidanemariam

  3. Department of Biotechnology, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia

    Adane D. Abraham

  4. Biosciences Eastern and Central Africa-International Livestock Research Institute (BecA-ILRI) Hub, Nairobi, Kenya

    Joyce N. Njuguna & Francesca Stomeo

Authors
  1. Dawit B. Kidanemariam
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  2. Amit C. Sukal
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  3. Adane D. Abraham
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  4. Joyce N. Njuguna
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  5. Francesca Stomeo
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  6. James L. Dale
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  7. Robert M. Harding
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  8. Anthony P. James
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Correspondence to Robert M. Harding.

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Handling Editor: F. Murilo Zerbini.

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Table S1

Percent amino acid sequence identity of the P3-coding region in pairwise sequence comparisons of PuPV with selected members of the family Luteoviridae (XLSX 12 kb)

Table S2

Percent amino acid sequence identity of the P4-coding region in pairwise sequence comparisons of PuPV with selected members of the family Luteoviridae (XLSX 12 kb)

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Kidanemariam, D.B., Sukal, A.C., Abraham, A.D. et al. Molecular characterisation of a putative new polerovirus infecting pumpkin (Cucurbita pepo) in Kenya. Arch Virol 164, 1717–1721 (2019). https://doi.org/10.1007/s00705-019-04219-8

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  • DOI: https://doi.org/10.1007/s00705-019-04219-8

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