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First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China

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Abstract

Enzootic nasal adenocarcinoma (ENA) of goats, characterized by transformation of epithelial cells of the ethmoid turbinates, is caused by enzootic nasal tumor virus 2 (ENTV-2). ENTV-2 belongs to the genus Betaretrovirus and has extended its distribution globally with a high prevalence; however, the genetic diversity and genotypic distribution for ENTV-2 have not been analyzed systematically due to the limited availability of sequence data. In this study, an infection by ENTV-2 was detected by RT-PCR in Chongqing in July 2018, and the complete sequence of one strain (CQ1) was determined. Phylogenetic analysis indicated a high degree of genetic heterogeneity among ENTV-2 sequences, with the existence of two main lineages. Lineage 1 and 2 were composed of ENTV-2 from China and the UK, respectively. Although CQ1 was closely related to recent ENTV-2 strains collected in the neighboring provinces of Chongqing (Shaanxi and Sichuan), it formed a separate sublineage of lineage 1 (sublineage 1.3). This report will enhance our understanding of the epidemiology of ENTV-2 in China.

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Funding

This work was supported by the National Key Research and Development Program of China (2018YFD0500500), the Chongqing Science & Technology Commission (cstc2018jscx-msybX0302, cstc2018jscx-msybX0221), the earmarked fund for China Agriculture Research System (CARS-37), the Fundamental Research Funds for the Central Universities (XDJK2017A003, SWU118072), and the Innovation Team Building Program in Chongqing Universities (CXTDG201602004).

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  1. Chao Ye and Qingyuan Huang contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Southwest University, No. 2 Tiansheng Road, Beibei District, Chongqing, 400715, China

    Chao Ye, Qingyuan Huang, Tingting Chen, Jiali Jiang, Fengqing Hou, Dongyi Xu, Yuanyi Peng & Rendong Fang

  2. Chongqing Academy of Animal Sciences, No. 238 Renmin Road, Yuzhong district, Chongqing, 402460, China

    Jing Chen

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  1. Chao Ye
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  2. Qingyuan Huang
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  9. Jing Chen
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Corresponding authors

Correspondence to Rendong Fang or Jing Chen.

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Supplementary material 1 (DOCX 115 kb)

Fig. S1

Pairwise comparisons between CQ1 and other strains based on the whole genome sequence (A) and an alignment of sequences of representative ENTV-2 strains based on the ORFx amino acid sequence (B). (JPEG 379 kb)

Supplementary material 3 (DOCX 14 kb)

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Ye, C., Huang, Q., Chen, T. et al. First detection and genotypic analysis of goat enzootic nasal tumor virus 2 in Chongqing, China. Arch Virol 164, 1647–1650 (2019). https://doi.org/10.1007/s00705-019-04211-2

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  • DOI: https://doi.org/10.1007/s00705-019-04211-2

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