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Novel bird’s-foot trefoil RNA viruses provide insights into a clade of legume-associated enamoviruses and rhabdoviruses

Archives of Virology volume 164, pages 1419–1426 (2019)Cite this article

Abstract

Here, we report the identification and characterization of two novel viruses associated with bird’s-foot trefoil. Virus sequences related to those of enamoviruses (ssRNA (+); Luteoviridae; Enamovirus) and nucleorhabdoviruses (ssRNA (-); Rhabdoviridae; Nucleorhabdovirus) were detected in Lotus corniculatus transcriptome data. The genome of the tentatively named “bird’s-foot trefoil-associated virus 1” (BFTV-1) is a 13,626-nt-long negative-sense ssRNA. BFTV-1 encodes six predicted gene products in the antigenome orientation in the canonical order 3′-N-P-P3-M-G-L-5′. The genome of the proposed “bird’s-foot trefoil-associated virus 2” (BFTV-2) is 5,736 nt long with a typical 5΄-PO-P1-2-IGS-P3-P5-3′ enamovirus genome structure. Phylogenetic analysis indicated that BFTV-1 is closely related to datura yellow vein nucleorhabdovirus and that BFTV-2 clusters into a monophyletic lineage of legume-associated enamoviruses. This subclade of highly related and co-divergent legume-associated viruses provides insights into the evolutionary history of the enamoviruses.

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Acknowledgements

We would like to express sincere gratitude to the generators of the underlying data used for this work: Dr. Ying Wang and Dr. Zhezhi Wang. By following open access practices and making accessible raw sequence data in public repositories available to the research community, they have promoted the generation of new knowledge and ideas.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sector.

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Authors and Affiliations

  1. Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria (IPAVE-CIAP-INTA), X5020ICA, Córdoba, Argentina

    Humberto J. Debat & Nicolas Bejerman

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina

    Nicolas Bejerman

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  1. Humberto J. Debat
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  2. Nicolas Bejerman
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Correspondence to Humberto J. Debat or Nicolas Bejerman.

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Debat, H.J., Bejerman, N. Novel bird’s-foot trefoil RNA viruses provide insights into a clade of legume-associated enamoviruses and rhabdoviruses. Arch Virol 164, 1419–1426 (2019). https://doi.org/10.1007/s00705-019-04193-1

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  • DOI: https://doi.org/10.1007/s00705-019-04193-1