Advertisement

Archives of Virology

, Volume 164, Issue 4, pp 1225–1228 | Cite as

Complete genome sequence of a new botybirnavirus isolated from a phytopathogenic Alternaria alternata in China

  • Guoping Ma
  • Zhijian Liang
  • Huihui Hua
  • Tao Zhou
  • Xuehong WuEmail author
Annotated Sequence Record
  • 108 Downloads

Abstract

A new double-stranded RNA (dsRNA) virus named Alternaria alternata botybirnavirus 1 (AaBRV1) was isolated from Alternaria alternata strain SD-BZF-19, a phytopathogenic fungus infecting watermelon in China. The genome of AaBRV1 consists of two dsRNA segments (dsRNAs 1 and 2), 6,130 and 5,862 bp in size, respectively. The sequence contains two putative open reading frames (ORFs) which encode two polyproteins of 1,874 and 1,784 amino acids, respectively. Nucleotide sequence comparisons revealed that the two ORFs of AaBRV1 have the highest similarity 60.3% and 56.7%, respectively, with dsRNAs 1 and 2 of Botrytis porri RNA virus 1 (BpRV1). The two polyproteins encoded by dsRNA1 and dsRNA2 shared the highest amino acid identities with the cap-pol fusion protein (60.2%) and hypothetical protein (53.7%) of BpRV1, respectively. AaBRV1 is composed of isometric particles, approximately 35 nm in diameter. Phylogenetic analysis of the RNA dependent RNA polymerase (RdRp) domain of the polyprotein revealed that AaBRV1 clusters together with members of the genus Botybirnavirus. These findings support the discovery of a new botybirnavirus in A. alternata.

Notes

Funding

This work was financially supported by Beijing Innovation Consortium of Melon Research System (Grant No. BAIC10-2019).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study did not include experiments with human participants or animals performed by any of the authors.

Informed consent

Informed consent was obtained from all individual participants included in this study.

Supplementary material

705_2019_4189_MOESM1_ESM.tif (4.9 mb)
Alignment of the 5´-terminal (A) and 3´-terminal (B) sequences of dsRNA1 and dsRNA2 of AaBRV1. Blue shading indicates identical nucleotides. The “●” symbol represents a missing nucleotide. The start codons (ATG) of ORF1 and ORF2 at the 5´-terminal are indicated with a red bar below the two nucleotide sequences (TIFF 5059 kb)
705_2019_4189_MOESM2_ESM.tif (255 kb)
Phylogenetic analysis of the polyproteins of AaBRV1 and selected dsRNA viruses of the genera Chrysovirus, Totivirus, Victorivirus, Botybirnavirus, and some unassigned dsRNA viruses using the neighbor-joining algorithm in MEGA 5.0. Bootstrap values lower than 50% are not shown. The scale bar represents a genetic distance of 0.2, and the red star indicates the position of AaBRV1 (TIFF 255 kb)
705_2019_4189_MOESM3_ESM.doc (46 kb)
Supplementary material 3 (DOC 46 kb)
705_2019_4189_MOESM4_ESM.doc (34 kb)
Supplementary material 4 (DOC 34 kb)

References

  1. 1.
    Xie JT, Jiang DH (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68CrossRefGoogle Scholar
  2. 2.
    Ghabrial SA, Castón JR, Jiang DH, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368CrossRefGoogle Scholar
  3. 3.
    Xiang J, Fu M, Hong N, Zhai LF, Xiao F, Wang GP (2017) Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus. Arch Virol 162:3907–3911CrossRefGoogle Scholar
  4. 4.
    Ran HC, Liu LJ, Li B, Cheng JS, Fu YP, Jiang DH, Xie JT (2016) Co-infection of a hypovirulent isolate of Sclerotinia sclerotiorum with a new botybirnavirus and a strain of a mitovirus. Virol J 13:92CrossRefGoogle Scholar
  5. 5.
    Wu MD, Jin FY, Zhang J, Yang L, Jiang DH, Li GQ (2012) Characterization of a novel bipartite double-stranded RNA mycovirus conferring hypovirulence in the phytopathogenic fungus Botrytis porri. J Virol 86:6605–6619CrossRefGoogle Scholar
  6. 6.
    Liu LJ, Wang QH, Cheng JS, Fu YP, Jiang DH, Xie JT (2015) Molecular characterization of a bipartite double-stranded RNA virus and its satellite-like RNA co-infecting the phytopathogenic fungus Sclerotinia sclerotiorum. Front Microbiol 6:406Google Scholar
  7. 7.
    Wang HR, Li C, Cai LN, Fang SG, Zheng LM, Yan F, Zhang SB, Liu Y (2018) The complete genomic sequence of a novel botybirnavirus isolated from a phytopathogenic Bipolaris maydis. Virus Genes 54:733–736CrossRefGoogle Scholar
  8. 8.
    Brun S, Madrid H, van den Ende BG, Andersen B, Marinach-Patrice C, Mazier D, de Hoog GS (2013) Multilocus phylogeny and MALDI-TOF analysis of the plant pathogenic species Alternaria dauci and relatives. Fungal Biol 117:32–40CrossRefGoogle Scholar
  9. 9.
    Wang TY, Zhao J, Sun P, Wu XH (2014) Characterization of Alternaria species associated with leaf blight of sunflower in China. Eur J Plant Pathol 140:301–315CrossRefGoogle Scholar
  10. 10.
    Zheng HH, Zhao J, Wang TY, Wu XH (2015) Characterization of Alternaria species associated with potato foliar diseases in China. Plant Pathol 64:425–433CrossRefGoogle Scholar
  11. 11.
    Zhao J, Bao SW, Ma GP, Wu XH (2016) Characterization of Alternaria species associated with muskmelon foliar diseases in Beijing municipality of China. J Gen Plant Pathol 82:29–32CrossRefGoogle Scholar
  12. 12.
    Zhao J, Bao SW, Ma GP, Wu XH (2016) Characterization of Alternaria species associated with watermelon leaf blight in Beijing municipality of China. J Plant Pathol 98:135–138Google Scholar
  13. 13.
    Zhao J, Ma GP, Liu YY, Wu XH (2018) Alternaria species infecting potato in southern China. Can J Plant Pathol 40:312–317CrossRefGoogle Scholar
  14. 14.
    Aoki N, Moriyama H, Kodama M, Arie T, Teraoka T, Fukuhara T (2009) A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata. Virus Res 140:179–187CrossRefGoogle Scholar
  15. 15.
    Okada R, Ichinose S, Takeshita K, Urayama SI, Fukuhara T, Komatsu K, Arie T, Ishihara A, Egusa M, Kodama M, Moriyama H (2018) Molecular characterization of a novel mycovirus in Alternaria alternata manifesting two-sided effects: Down-regulation of host growth and up-regulation of host plant pathogenicity. Virology 519:23–32CrossRefGoogle Scholar
  16. 16.
    Xavier AdS, de Barros APO, Godinho MT, Zerbini FM, Souza FdO, Bruckner FP, Alfenas-Zerbini P (2018) A novel mycovirus associated to Alternaria alternata comprises a distinct lineage in Partitiviridae. Virus Res 244:21–26CrossRefGoogle Scholar
  17. 17.
    Morris TJ, Dodds JA (1979) Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858CrossRefGoogle Scholar
  18. 18.
    Lyu RL, Zhang Y, Tang Q, Li YY, Cheng JS, Fu YP, Chen T, Jiang DH, Xie JT (2018) Two alphapartitiviruses co-infecting a single isolate of the plant pathogenic fungus Rhizoctonia solani. Arch Virol 163:515–520CrossRefGoogle Scholar
  19. 19.
    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25:4876–4882CrossRefGoogle Scholar
  20. 20.
    Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28:2731–2739CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Plant PathologyChina Agricultural UniversityBeijingPeople’s Republic of China

Personalised recommendations