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The complete genome sequence of wild tomato mosaic virus isolated from Solanum nigrum reveals recombination in the P1 cistron

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Abstract

The complete genome sequence of a wild tomato mosaic virus (WTMV) isolate (named WTMV-Sn) was determined and identified in Solanum nigrum in China. The complete genome of WTMV-Sn is 9,659 nucleotides in length, excluding the poly(A) tail and encodes a polyprotein of 3,074 amino acids. This is the first report of WTMV infecting S. nigrum. Despite the high degree of sequence similarity between the WTMV-Sn and WTMV-XC-1 isolates, the 349 nucleotides at the 5′ terminus of WTMV-Sn appear to have originated by recombination with another isolate. The recombination parent remains unknown, but the recombination region shares 74.57% sequence identity with isolate WTMV-Laichau, which is below the species demarcation threshold for the genus Potyvirus. A pathogenicity test showed that WTMV-Sn can infect tobacco. This suggests that variation in the P1 cistron of WTMV-Sn may contribute to its ability to infect S. nigrum.

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Acknowledgements

We are very appreciative of Dr. Ralf Georg Dietzgen (Editor of Archives of Virology) for language editing. These studies were supported by the National Key Research and Development Plan, Project no. 2017YFD0301704, 2018YFD0201006, 2018YFD0200700; Research Foundation of Sichuan Provincial Education Department (035Z1038); Sichuan Provincial Science and Technology Project, Project no. 2016NYZ0053; Chengdu Science and Technology Project, Rapid Identification and Virus-Free Technology of Kiwifruit Virus.

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  1. Lei Zhang and Jing Shang contributed equally to this work.

Authors and Affiliations

  1. Sichuan Engineering Research Center for Crop Strip Intercropping System and Key Laboratory of Crop Eco-physiology and Farming System in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China

    Lei Zhang, Jing Shang, Qi Jia & Wenyu Yang

  2. College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, China

    Lei Zhang, Jing Shang, Qi Jia, Guoshu Gong & Min Zhang

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  1. Lei Zhang
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  2. Jing Shang
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  3. Qi Jia
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  4. Guoshu Gong
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  5. Min Zhang
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Correspondence to Jing Shang.

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Handling Editor: Ralf Georg Dietzgen.

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Zhang, L., Shang, J., Jia, Q. et al. The complete genome sequence of wild tomato mosaic virus isolated from Solanum nigrum reveals recombination in the P1 cistron. Arch Virol 164, 903–906 (2019). https://doi.org/10.1007/s00705-019-04146-8

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  • DOI: https://doi.org/10.1007/s00705-019-04146-8

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