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Characterization of a novel lytic myophage, phiA8-29, infecting Aeromonas strains

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Abstract

Members of the bacterial genus Aeromonas are important aquatic pathogens that cause severe fish diseases. Here, we characterize a novel lytic phage, Aeromonas virus phiA8-29, isolated from the alimentary tract of a freshwater fish. Transmission electron microscopy showed that phiA8-29 has a long contractile tail and thus can be classified as a member of the family Myoviridae. The phage genome was identified as a double-stranded DNA molecule of 144,974 bp containing 185 open reading frames and nine tRNA-encoding genes. Comparative genomic analysis revealed that the phiA8-29 genome has little similarity to any of the currently sequenced Aeromonas phage genomes. Our data indicate that phiA8-29 is a novel lytic Myoviridae phage that does not belong to any of the known genera.

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Acknowledgements

This work is supported by National Natural Science Foundation of China (Grant No. 31370205).

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Industrial Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China

    Yang He, Zhiwei Huang, Xixi Zhang, Zhiqiang Zhang, Mengxin Gong, Xuewei Pan & Hongjiang Yang

  2. State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, 300071, China

    Dongsheng Wei

Authors
  1. Yang He
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  2. Zhiwei Huang
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  3. Xixi Zhang
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  4. Zhiqiang Zhang
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  5. Mengxin Gong
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  6. Xuewei Pan
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  7. Dongsheng Wei
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  8. Hongjiang Yang
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Corresponding author

Correspondence to Hongjiang Yang.

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The authors declare no competing financial interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Chan-Shing Lin.

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He, Y., Huang, Z., Zhang, X. et al. Characterization of a novel lytic myophage, phiA8-29, infecting Aeromonas strains. Arch Virol 164, 893–896 (2019). https://doi.org/10.1007/s00705-018-4109-y

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  • DOI: https://doi.org/10.1007/s00705-018-4109-y

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