Skip to main content

Sequence analysis and genomic organization of a novel chuvirus, Tàiyuán leafhopper virus

Archives of Virology volume 164, pages 617–620 (2019)Cite this article

Abstract

A novel unsegmented chuvirus was found in a leafhopper (Psammotettix alienus) and tentatively named “Tàiyuán leafhopper virus” (TYLeV). The full genome of TYLeV is 14,670 nucleotides in length and includes four ORFs encoding glycoprotein, nucleocapsid protein, RNA-dependent RNA polymerase and an uncharacterized protein, respectively. These genomic features are similar to those of the chuvirus Scaldis River bee virus, but the virus is highly divergent from known chuviruses and other members in the order Mononegavirales, with amino acid sequence identities of only 13.6–33% for the RNA-dependent RNA polymerase (RdRp). On the basis of genomic features, phylogenetic analysis and comparisons of RdRp sequences, we suggest that this virus is a member of a new species of chuvirus in the proposed genus Mivirus.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1

References

  1. Abt I, Derlink M, Mabon R, Virant-Doberlet M, Jacquot E (2018) Integrating multiple criteria for the characterization of Psammotettix populations in European cereal fields. Bull Entomol Res 108:185–202

    Article  CAS  PubMed  Google Scholar 

  2. Afiahayati Sato K, Sakakibara Y (2015) MetaVelvet-SL: an extension of the Velvet assembler to a de novo metagenomic assembler utilizing supervised learning. DNA Res 22:69–77

    Article  CAS  PubMed  Google Scholar 

  3. Amarasinghe GK, Arechiga Ceballos NG, Banyard AC, Basler CF (2018) Taxonomy of the order Mononegavirales: update 2018. Arch Virol 163:2283–2294

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Dietzgen RG, Kondo H, Goodin MM, Kurath G, Vasilakis N (2017) The family Rhabdoviridae: mono- and bipartite negative-sense RNA viruses with diverse genome organization and common evolutionary origins. Virus Res 227:158–170

    Article  CAS  PubMed  Google Scholar 

  6. Garcia ML, Bo ED, da Graca JV, Gago-Zachert S, Hammond J, Moreno P, Natsuaki T, Pallas V, Navarro JA, Reyes CA, Luna GR, Sasaya T, Tzanetakis IE, Vaira AM, Verbeek M, Ictv Report C (2018) Corrigendum: ICTV Virus taxonomy profile: Ophioviridae. J Gen Virol 99:949

    Article  PubMed  PubMed Central  Google Scholar 

  7. Li CX, Shi M, Tian JH, Lin XD, Kang YJ, Chen LJ, Qin XC, Xu J, Holmes EC, Zhang YZ (2015) Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. eLife 4:e05378

    Article  CAS  PubMed Central  Google Scholar 

  8. Liu L, Xie J, Cheng J, Fu Y, Li G, Yi X, Jiang D (2014) Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses. Proc Natl Acad Sci USA 111:12205–12210

    Article  CAS  PubMed  Google Scholar 

  9. Liu X, Wu J, Wang J, Liu X, Zhao S, Li Z, Kong L, Gu X, Luo J, Gao G (2009) WebLab: a data-centric, knowledge-sharing bioinformatic platform. Nucleic Acids Res 37:W33–W39

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Liu Y, Du Z, Wang H, Zhang S, Cao M, Wang X (2018) Identification and characterization of wheat yellow striate virus, a novel leafhopper-transmitted nucleorhabdovirus infecting wheat. Front Microbiol 9:468

    Article  PubMed  PubMed Central  Google Scholar 

  11. Maes P, Alkhovsky SV, Bao Y, Beer M, Birkhead M (2018) Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018. Arch Virol 163:2295–2310

    Article  CAS  PubMed  Google Scholar 

  12. Olivier Poch IS, Delarue Marc, Tordo Noel (1989) Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO J 8:3867–3874

    Article  Google Scholar 

  13. Qin F, Liu W, Wu N, Zhang L, Zhang Z, Zhou X, Wang X (2018) Invasion of midgut epithelial cells by a persistently transmitted virus is mediated by sugar transporter 6 in its insect vector. PloS Pathog 14:e1007201

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Schoonvaere K, De Smet L, Smagghe G, Vierstraete A, Braeckman BP, de Graaf DC (2016) Unbiased RNA shotgun metagenomics in social and solitary wild bees detects associations with eukaryote parasites and new viruses. PloS One 11:e0168456

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Vacke J (1961) Wheat dwarf virus disease. Biol Plant 3:228–233

    Article  Google Scholar 

  16. Wang Y, Mao Q, Liu W, Mar T, Wei T, Liu Y, Wang X (2014) Localization and distribution of wheat dwarf virus in its vector leafhopper, Psammotettix alienus. Phytopathology 104:897–904

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This research was supported by the Inter-Governmental S&T Cooperation Proposal (2016YFE0131000) and the National Key Research and Development Program of China (2017YFD0201700).

Author information

Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Hui Wang, Yan Liu, Wenwen Liu & Xifeng Wang

  2. National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China

    Mengji Cao

Authors
  1. Hui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wenwen Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mengji Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xifeng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yan Liu.

Ethics declarations

Conflict of interest

The authors declare no conflict of interest.

Ethical statement

No experimental work with humans was done in this study.

Additional information

Handling Editor: Tim Skern.

Electronic supplementary material

Below is the link to the electronic supplementary material.

705_2018_4075_MOESM1_ESM.jpg

Fig. S1 Amino acid sequence alignment of core RdRp motifs of TYLeV, chuviruses, and selected viruses of the order Mononegavirales (JPEG 341 kb)

Supplementary material 2 (DOCX 14 kb)

Supplementary material 3 (DOCX 19 kb)

Supplementary material 4 (fa 14 kb)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Wang, H., Liu, Y., Liu, W. et al. Sequence analysis and genomic organization of a novel chuvirus, Tàiyuán leafhopper virus. Arch Virol 164, 617–620 (2019). https://doi.org/10.1007/s00705-018-4075-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-018-4075-4