Griffithsin inhibits porcine reproductive and respiratory syndrome virus infection in vitro
- 189 Downloads
Porcine reproductive and respiratory syndrome virus (PRRSV) is a pathogen that severely disrupts swine production. Despite sustained efforts, the disease is still endemic, with high mortality and morbidity. New antiviral strategies to control PRRSV are needed. Griffithsin, a red algal lectin, has potent antiviral effect on several human enveloped viruses, but this effect has not been demonstrated on PRRSV. Here, we first tested the in vitro antiviral activity of Griffithsin against PRRSV. Griffithsin exerted strong saccharide-dependent antiviral activity against PRRSV, probably through interactions with glycans on the surface of PRRSV that interfered with virus entry. Furthermore we revealed that Griffithsin’s action on PRRSV involved blocking viral adsorption, and it had no effect on viral penetration. Besides Our findings also suggested that Griffithsin may interfere with cell-to-cell spread to prevent virus transmission. The remarkable potency profile of Griffithsin supports its potential value as an antiviral agent against PRRSV.
This study was supported by grants from the Special Fund for Agro-scientific Research in the Public Interest (No. 201303046). The erythrocytes were kindly provided by Yiwei Wang.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- 1.Kristensen C, Kvisgaard L, Pawlowski M, Holmgaard Carlsen S, Hjulsager C, Heegaard P, Bøtner A, Stadejek T, Haugegaard S, Larsen L (2017) Efficacy and safety of simultaneous vaccination with two modified live virus vaccines against porcine reproductive and respiratory syndrome virus types 1 and 2 in pigs. Vaccine 36(2):227–236CrossRefGoogle Scholar
- 8.Xue J (2014) Investigation of the mechanism of Griffithsin (GRFT): a potent HIV entry inhibitor. Dissertations & Theses-GradworksGoogle Scholar
- 10.O’Keefe BR, Giomarelli B, Barnard DL, Shenoy SR, Chan PKS, Mcmahon JB, Palmer KE, Barnett BW, Meyerholz DK, Wohlfordlenane CL (2010) Broad-spectrum in vitro activity and in vivo efficacy of the antiviral protein Griffithsin against emerging viruses of the family Coronaviridae. J Virol 84:2511–2521CrossRefGoogle Scholar
- 19.Kouokam JC, Huskens D, Schols D, Johannemann A, Riedell SK, Walter W, Walker JM, Matoba N, O’Keefe BR, Palmer KE (2011) Investigation of Griffithsin’s interactions with human cells confirms its outstanding safety and efficacy profile as a microbicide candidate. PLoS One 6:e22635CrossRefGoogle Scholar