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Archives of Virology

, Volume 163, Issue 12, pp 3373–3376 | Cite as

Bottlenecks and complementation in the aphid transmission of citrus tristeza virus populations

  • S. J. HarperEmail author
  • S. J. Cowell
  • W. O. Dawson
Brief Report
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Abstract

Aphid transmission is a major factor in the formation of citrus tristeza virus (CTV) populations. Here, we examined the effect of population interaction on aphid transmissibility of different CTV genotypes. We found that there was no correlation between the proportion of viral genotypes in the source population and what was transmitted. We next examined the transmission of a poorly transmitted infectious cDNA clone (T36) in mixture with other CTV genotypes. T36 transmission increased from 0.5% alone, to up to 35.7%, depending on the coinfecting genotype. These results suggest that interaction between CTV genotypes affects the transmission of this virus.

Notes

Acknowledgements

The authors wish to acknowledge the contributions made by the late S. Garnsey, not only in this study, but also to the field of citrus virology as a whole. The authors also wish to acknowledge the assistance of T. Shilts and J. Capps in performing the aphid transmissions, and C. Bierman for performing the ELISA assays. This research was supported in part by an endowment from the J.R. and Addie S. Graves family and the Florida Agricultural Experiment Station.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

References

  1. 1.
    Betancourt M, Fereres A, Fraile A, García-Arenal F (2008) Estimation of the effective number of founders that initiate an infection after aphid transmission of a multipartite plant virus. J Virol 82(24):12416–12421CrossRefGoogle Scholar
  2. 2.
    Moreno P, Ambros S, Albiach-Marti MR, Guerri J, Pena L (2008) Citrus tristeza virus: a pathogen that changed the course of the citrus industry. Mol Plant Pathol 9(2):251–268CrossRefGoogle Scholar
  3. 3.
    Ayllón MA, Rubio L, Moya A, Guerri J, Moreno P (1999) The haplotype distribution of two genes of Citrus tristeza virus is altered after host change or aphid transmission. Virology 255(1):32–39CrossRefGoogle Scholar
  4. 4.
    Broadbent P, Brlansky R, Indsto J (1996) Biological characterization of Australian isolates of Citrus tristeza virus and separation of subisolates by single aphid transmissions. Plant Dis 80(3):329–333CrossRefGoogle Scholar
  5. 5.
    Brlansky RH, Damsteegt VD, Howd DS, Roy A (2003) Molecular analyses of Citrus tristeza virus subisolates separated by aphid transmission. Plant Dis 87(4):397–401CrossRefGoogle Scholar
  6. 6.
    Moreno P, Guerri J, Ballester-Olmos JF, Albiach R, Martínez ME (1993) Separation and interference of strains from a Citrus tristeza virus isolate evidenced by biological activity and double-stranded RNA (dsRNA) analysis. Plant Pathol 42(1):35–41CrossRefGoogle Scholar
  7. 7.
    Albiach-Martí MR, Guerri J, de Mendoza AH, Laigret F, Ballester-Olmos JF, Moreno P (2000) Aphid transmission alters the genomic and defective RNA populations of Citrus tristeza virus isolates. Phytopathology 90(2):134–138CrossRefGoogle Scholar
  8. 8.
    Roy A, Brlansky RH (2009) Population dynamics of a Florida Citrus tristeza virus isolate and aphid-transmitted subisolates: identification of three genotypic groups and recombinants after aphid transmission. Phytopathology 99(11):1297–1306CrossRefGoogle Scholar
  9. 9.
    Harper SJ, Cowell SJ, Dawson WO (2015) With a little help from my friends: complementation as a survival strategy for viruses in a long-lived host system. Virology 478:123–128CrossRefGoogle Scholar
  10. 10.
    Harper SJ, Cowell SJ, Dawson WO (2017) Isolate fitness and tissue-tropism determine superinfection success. Virology 511:222–228CrossRefGoogle Scholar
  11. 11.
    Killiny N, Harper SJ, Alfaress S, El Mohtar C, Dawson WO (2016) Minor coat and heat shock proteins are involved in the binding of Citrus tristeza virus to the foregut of its aphid vector, Toxoptera citricida. Appl Environ Microbiol 82(21):6294–6302CrossRefGoogle Scholar
  12. 12.
    Harper SJ, Killiny N, Tatineni S, Gowda S, Cowell SJ, Shilts T, Dawson WO (2016) Sequence variation in two genes determines the efficacy of transmission of Citrus tristeza virus by the brown citrus aphid. Arch Virol 161(12):3555–3559CrossRefGoogle Scholar
  13. 13.
    Harper SJ (2013) Citrus tristeza virus: evolution of complex and varied genotypic groups. Front Microbiol 4:93CrossRefGoogle Scholar
  14. 14.
    Satyanarayana T, Bar-Joseph M, Mawassi M, Albiach-Martí MR, Ayllón MA, Gowda S, Hilf ME, Moreno P, Garnsey SM, Dawson WO (2001) Amplification of Citrus tristeza virus from a cDNA clone and infection of citrus trees. Virology 280(1):87–96CrossRefGoogle Scholar
  15. 15.
    Froissart R, Michalakis Y, Blanc S (2002) Helper component-transcomplementation in the vector transmission of plant viruses. Phytopathology 92(6):576–579CrossRefGoogle Scholar
  16. 16.
    Tian T, Rubio L, Yeh HH, Crawford B, Falk BW (1999) Lettuce infectious yellows virus: in vitro acquisition analysis using partially purified virions and the whitefly Bemisia tabaci. J Gen Virol 80:1111–1117CrossRefGoogle Scholar
  17. 17.
    Alzhanova DV, Prokhnevsky AI, Peremyslov VV, Dolja VV (2007) Virion tails of Beet yellows virus: coordinated assembly by three structural proteins. Virology 359:220–226CrossRefGoogle Scholar
  18. 18.
    Bonnet J, Fraile A, Sacristán S, Malpica JM, García-Arenal F (2005) Role of recombination in the evolution of natural populations of Cucumber mosaic virus, a tripartite RNA plant virus. Virology 332(1):359–368CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Plant PathologyWashington State UniversityProsserUSA
  2. 2.Citrus Research and Education CenterUniversity of FloridaLake AlfredUSA

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