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The complete genomic sequence of a novel megabirnavirus from Fusarium pseudograminearum, the causal agent of wheat crown rot

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A novel double-stranded RNA (dsRNA) mycovirus, named Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1), was found in a hypovirulent strain FC136-2A of Fusarium pseudograminearum, the causal agent of wheat crown rot. The complete genome of FpgMBV1 comprised two dsRNA segments, L1-dsRNA (8951 bp) and L2-dsRNA (5337 bp). Both dsRNAs carry two open reading frames on the positive strand. L1-dsRNA potentially encodes a 131.3-kDa coat protein (CP) and a 126.3-kDa RNA-dependent RNA polymerase (RdRp), while L2-dsRNA encodes two putative proteins of 97.2 kDa and 30.6 kDa with unknown functions. As found in members of the family Megabirnaviridae, the 5’- and 3’-terminal sequences of L1-dsRNA and L2-dsRNA of FpgMBV1 are highly conserved. The identity between L1-dsRNA (1630 nt) and L2-dsRNA (1682 nt) is 88.95%, and the first 236 nt (nt 1-236) are exactly identical. The 3’ UTRs of L1-dsRNA (155 nt) and L2-dsRNA (109 nt) are 66.67% identical, with identical terminal hexamer sequences (5’-AAAAGC-3’). Phylogenetic analyses based on the amino acid sequences of the RdRps indicated that FpgMBV1 is a new member of the family Megabirnaviridae.

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This research was financially supported by the National Special Fund for Agro-Scientific Research in the Public Interest of China (201503112) and Henan Province Agricultural Science and Technology Research Project (182102110004). The authors would like to thank Prof. Jiatao Xie for advice during the preparation of the manuscript.

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Correspondence to Honglian Li.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Handling Editor: Massimo Turina.

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Supplemental Figure 1 Comparison of the 5’- and 3’- termini of the two dsRNA segments of FpgMBV1. (PDF 56 kb)


Supplemental Figure 2 Phylogenetic analysis of FpgMBV1 and selected members of the families Megabirnaviridae, Chrysoviridae, Quadriviridae, Totiviridae and Botybirnaviridae based on the aligned RdRp aa sequences of the viruses by the neighbor-joining method using the program MEGA 7.0. FpgMBV1 is highlighted in red with filled circle; other viruses in Megabirnaviridae are highlighted in black with filled circle; viruses in Chrysoviridae are highlighted in square; viruses in Quadriviririda are highlighted in diamond; viruses in Totiviridae are highlighted in simple circle and viruses in Botybirnaviridae are highlighted in triangle. Bootstrap values (1000 replicates) are shown at the nodes. The scale bar (0.1) corresponds to genetic distance. (PDF 46 kb)

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Zhang, X., Gao, F., Zhang, F. et al. The complete genomic sequence of a novel megabirnavirus from Fusarium pseudograminearum, the causal agent of wheat crown rot. Arch Virol 163, 3173–3175 (2018).

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